Part I. Introduction: 1. Anatomy and physiology of the male reproductive system Ryan Scribner and Nick N. Tadros; 2. Evaluation of candidates for sperm retrieval Darren J. Bryk, Neel V. Parekh and Sarah C. Vij; 3. Testicular histopathology and the role of testis biopsy Gianmartin Cito; Part II. Sperm Retrieval: 4. History of surgical sperm retrieval techniques Rupin Shah; 5. Epididymal sperm retrieval: indications, surgical protocol, and outcomes Jason P. Akerman and R. Matthew Coward; 6. Testicular sperm retrieval: indications, surgical protocol, and outcomes Ahmad Majzoub, Sandro C. Esteves and Chak-Lam Cho; 7. Surgical and non-surgical sperm retrieval techniques in patients with ejaculatory dysfunctions Mohamed Adnan, Omer Raheem and Laith M. Alzweri; 8. Sperm retrieval in non-azoospermic men: indications, protocol, and outcomes Sandro C. Esteves and Ahmad Majzoub; 9. Predictors of positive surgical sperm retrieval in azoospermic males: evaluation of clinical, laboratory, and histopathologic factors Kareim Khalafalla, Ahmed H. Almalki, Ahmad Majzoub and Sandro C. Esteves; 10. Methods for enhancing surgical sperm retrieval success rate Jordan Cohen and Ranjith Ramasamy; Part III. Laboratory Handling of Retrieved Sperm: 11. Critical factors for optimizing sperm handling and ICSI outcomes Edson Borges, Amanda Souza Setti and Daniela Paes de Almeida Ferreira Braga; 12. Sperm cryopreservation Rakesh Sharma, Kruyanshi Master and Ashok Agarwal; 13. Future directions in sperm handling for ART Manesh Panner Selvam Kumar, Catalina Barbarosie and Ashok Agarwal.