Mammalian Synthetic Promoters

ISBN-13: 9781493972210 / Angielski / Twarda / 2017 / 277 str.

David Gould

Opis

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application.

Spis treści

1.         Initial Considerations before Designing a Promoter Construct

David Gould

 

Part I: Monitoring Promoter Interactions and Activity

2          Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay

Nasim Yousaf and David Gould

 

3.         Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter

Jordan Read

 

4.         Secreted Reporters for Monitoring Multiple Promoter Function

Ghazal Lashgari, Rami S. Kantar, and Bakhos A. Tannous

 

5.         Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo

Juliette MKM Delhove, Rajvinder Karda, Kate E Hawkins, Simon N Waddington, and Tristan R McKay

 

6.         Monitoring Promoter Activity by Flow Cytometry

Taher E. I. Taher

 

Part II: Promoter Design and Applications

7.         Functional Screening of Core Promoter Activity

Dan Y. Even, Adi Kedmi, Diana Ideses, and Tamar Juven-Gershon

 

8.         Bioinformatically Informed Design of Synthetic Mammalian Promoters

Michael L. Roberts, Polyxeni Katsoupi, Vivian Tsevleki, and Era Taoufik

 

9.         Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication

M. Veronica Lopez, Eduardo. G. Cafferata, Diego. L. Viale, and Osvaldo Podhajcer

 

10.       Constructing Strong Cell-Type-Specific Promoters through Informed Design

Adam J. Brown and David C. James

 

11.       PCR Assembly of Synthetic Promoters

Hodan Mohamed and David Gould

 

Part III: Regulated Promoter Approaches

12.       The Tetracycline Responsive System

Nasim Yousaf and David Gould

 

13.       Light-responsive Promoters

Maximilian Hörner, Konrad Müller, and Wilfried Weber

 

14.       A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation

Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, and Takashi Kondo

15.       Promoter Activation with Electromagnetism

Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, and Reyes S. Taméz-Guerra

 

16.       Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment

Eduardo G Cafferata, M Verónica López, Felipe J Nuñez, María Agostina Russo Maenza, and Osvaldo L Podhajcer

 

Part IV: Combining Promoters in Synthetic Circuits

17.       A Modular Approach to Building Complex Synthetic Circuits

Yinqing Li and Ron Weiss

 

18.       Computational Sequence Design with R2oDNA Designer

James T. MacDonald and Velia Siciliano

 

19.       Design of Synthetic Promoters for Gene Circuits in Mammalian Cells

Pratik Saxena, Daniel Bojar, and Martin Fussenegger

Blurb

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.