Mammalian Cell Viability: Methods and Protocols

ISBN-13: 9781617791079 / Angielski / Twarda / 2011 / 254 str.

Martin J. Stoddart

Opis

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Spis treści

1. Cell Viability Assays: Introduction

            Martin J. Stoddart

 

2. Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

            Kristine S. Louis and Andre C. Siegel

 

3. Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay

            László Kupcsik

 

4. WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

            Martin J. Stoddart

 

5. Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

            Ewa M. Czekanska

 

6. The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability

            Ning Ke, Xiaobo Wang, Xiao Xu, and Yama A. Abassi

 

7. Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays

            Caroline Woolston and Stewart Martin

 

8. Annexin V / 7-AAD Staining in Keratinocytes

            Maya Zimmermann and Norbert Meyer

 

9. Measurement of Caspase Activity: From Cell Populations to Individual Cells

            Gabriela Paroni and Claudio Brancolini

 

10. Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

            Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz

 

11. Multiplexing Cell Viability Assays

            Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar

 

12. Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death

            Terry L. Riss, Richard A. Moravec, and Andrew L. Niles

 

13. Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs

            Heather M. Powell, Alexis D. Armour, and Steven T. Boyce

 

14. Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers

            Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, and Sibylle Grad

 

15. Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections

            Katharina Jähn and Martin J. Stoddart

 

16. Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay

            Won-Kyu Ju and Keun-Young Kim

 

17. Exploiting the Liberation of Zn²⁺ to Measure Cell Viability

            Christian J. Stork and Yang V. Li

 

18. Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

            Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou

 

19. Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test

            Sophie Verrier, Alina Zoladek, and Ioan Notingher

 

20. Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses

            Olivier Braissant and Alma U. “Dan” Daniels

 

21. Digital Image Processing of Live/Dead Staining

            Pieter Spaepen, Sebastian De Boodt, Jean-Marie Aerts, and Jos Vander Sloten