Magnaporthe oryzae: Methods and Protocols

ISBN-13: 9781071616154 / Angielski / Miękka / 2022

Jacob, Stefan

Opis

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen’s adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field. 

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. The Impact of Blast Disease: Past, Present, and Future
Barbara Valent

2. The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae
Neftaly Cruz-Mireles, Iris Eisermann, Marisela Garduño-Rosales, Camilla Molinari, Lauren S. Ryder, Bozeng Tang, Xia Yan, and Nicholas J. Talbot

3. Analysis of the Secondary Metabolism in Magnaporthe oryzae
Elizabeth Skellam

4. The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD)
Christiane Grünewald, Stefan Bohnert, and Stefan Jacob

5. Characterizing the Secretion Systems of Magnaporthe oryzae
Ely Oliveira-Garcia and Barbara Valent

6. Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy
Raquel O. Rocha and Richard A. Wilson 

7. 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae
Audra Rogers, Nawaraj Dulal, and Martin Egan

8. Tandem Affinity Purification (TAP) of Low Abundance Protein Complexes in Filamentous Fungi Demonstrated using Magnaporthe oryzae
Gang Li and Richard A. Wilson 

9. Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae
Thomas Michna and Stefan Tenzer

10. A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway
Stefan Bohnert, Hendrik Neumann, and Stefan Jacob

11. Making Visualization Work for You: Deriving Valuable Insights from Omics Data
Alexander Yemelin

12. Genome Editing using CRISPR/Cas9 System in the Rice Blast Fungus
Takayuki Arazoe

13. Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae
Ciarán Griffin and George R. Littlejohn

14. Methods to Study Autophagocytosis in Magnaporthe oryzae
Lin Li, Xue-Ming Zhu, Xiao-Hong Liu, and Fu-Cheng Lin

15. Molecular Detection of Magnaporthe oryzae from Rice Seeds
Sonia Chadha

16. Isolation and Functional Analysis of Effector-Proteins of Magnaporthe oryzae
Meng-Di Dai, Yu Li, Li-Xiao Sun, Fu-Cheng Lin, and Xiao-Hong Liu

17. Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae
Sonia Chadha

18 Controllable Bypass Suppression in Magnaporthe oryzae
Stefan Jacob and Katharina Bersching

Subject Index List…