Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook
21. Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections
Dominik Ruckerl
22. Genetic Models of Macrophage Depletion
Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren
23. Depleting Macrophages In Vivo with Clodronate-Liposomes
Stéphanie G. Moreno
24. Cre Driver Mice Targeting Macrophages
Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren
This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.
The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.