Lymphoma: Methods and Protocols » książka
Lymphoma: Methods and Protocols
ISBN-13: 9781627032681 / Angielski / Twarda / 2013 / 315 str.
Lymphoma: Methods and Protocols
ISBN-13: 9781627032681 / Angielski / Twarda / 2013 / 315 str.
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Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.
Wydanie ilustrowane
1. Origin and Pathogenesis of B Cell Lymphomas
Marc Seifert, René Scholtysik, and Ralf Küppers
2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma
David Wu, Brent L. Wood, and Jonathan R. Fromm
3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells
Ralf Küppers, Markus Schneider, and Martin-Leo Hansmann
4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics
Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group
5. Expression Cloning of Human B Cell Immunoglobulins
Hedda Wardemann and Juliane Kofer
6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR
Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen
7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies
Martin J.S. Dyer
8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
Nikos Darzentas and Kostas Stamatopoulos
9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
Sebastian Böttcher, Matthias Ritgen, and Michael Kneba
10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR
Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba
11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass
12. Gene Expression Profile Analysis of Lymphomas
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Martin J.S. Dyer
8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
Nikos Darzentas and Kostas Stamatopoulos
9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
Sebastian Böttcher, Matthias Ritgen, and Michael Kneba
10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR
Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba
11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass
12. Gene Expression Profile Analysis of Lymphomas
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group
5. Expression Cloning of Human B Cell Immunoglobulins
Hedda Wardemann and Juliane Kofer
6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR
Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen
7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies
Martin J.S. Dyer
8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
Nikos Darzentas and Kostas Stamatopoulos
9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
Sebastian Böttcher, Matthias Ritgen, and Michael Kneba
10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR
Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba
11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass
12. Gene Expression Profile Analysis of Lymphomas
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Martin J.S. Dyer
8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas
Nikos Darzentas and Kostas Stamatopoulos
9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies
Sebastian Böttcher, Matthias Ritgen, and Michael Kneba
10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR
Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba
11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass
12. Gene Expression Profile Analysis of Lymphomas
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Katia Basso and Ulf Klein
13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Maciej Giefing and Reiner Siebert
14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens
Vu N. Ngo
15. Studying MicroRNAs in Lymphoma
Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg
16. Molecular Methods of Virus Detection in Lymphoma
Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer
17. High Throughput RNA Sequencing in B-Cell Lymphomas
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz
Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.
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