Lymphoma: Methods and Protocols

ISBN-13: 9781493991501 / Angielski / Twarda / 2019 / 439 str.

Ralf Kuppers

Spis treści

Preface...

Table of Contents...

Contributing Authors...

1. Origin and Pathogenesis of B Cell Lymphomas

Marc Seifert, René Scholtysik, and Ralf Küppers

2. Flow Cytometry for Non-Hodgkin and Hodgkin Lymphoma

Emily Glynn, Lori Soma, David Wu, Brent L. Wood, and Jonathan R. Fromm

3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells

Ralf Küppers, Markus Schneider, and Martin-Leo Hansmann

4. PCR GeneScan and Heteroduplex Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations 

Elke Boone, Kim C. Heezen, Patricia JTA Groenen, and Anton W. Langerak

5. Expression Cloning of Antibodies from Single Human B Cells

Hedda Wardemann and Christian E. Busse

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

Menno C. van Zelm 

7. Stereotyped B Cell Immunoglobulins in B Cell Lymphomas

Andreas Agathangelidis, Fotis Psomopoulos, and Kostas Stamatopoulos 

8. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

Sebastian Böttcher

9. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

10. Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input

Dieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, and Christoph Plass

11. FISH and FICTION in Lymphoma Research

Maciej Giefing and Reiner Siebert

12. NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma 

Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, and Agnieszka Dzikiewicz-Krawczyk

13. RNA Sequencing in B-Cell Lymphomas

Da Wei Huang, Moez Dawood, Calvin A. Johnson, and Roland Schmitz

14. Studying Cancer Heterogeneity by Single Cell RNA-Sequencing 

Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, and Wolfgang Enard

15. Evaluation of Whole Genome Sequencing Data

Daniel Huebschmann and Matthias Schlesner

16. Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines

Daniel E. Webster, Sandrine Roulland, and James Phelan

17. Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens 

Katarzyna Tomska, Sebastian Scheinost, and Thorsten Zenz

18. Proximity Ligation Assay

Ryan M. Young

19. Phospoproteomic Analysis of Signaling Pathways in Lymphomas

Björn Häupl, Henning Urlaub, and Thomas Oellerich

20. Ultrasensitive Detection of Circulating Tumour DNA in Lymphoma via Targeted Hybridization Capture and Deep Sequencing of Barcoded Libraries

Miguel Alcaide, Christopher Rushton, and Ryan D. Morin

Blurb

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Innovative and thorough, Lymphoma: Methods and Protocols, Second Edition is a valuable resource for hematologists, hematopathologists, and any scientists interested in cancer research, human genetics, and immunology.