Live Cell Imaging: Methods and Protocols

ISBN-13: 9781071612576 / Angielski / Twarda / 2021 / 447 str.

Sung-Bae Kim

Spis treści

Part I: Imaging with Passive Optical Readouts

 

1. Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins

            Toshiyuki Taniyama and Shinji Sueda

 

2. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools

            Osamu Takenouchi, Hideaki Yoshimura, and Takeaki Ozawa

 

3. Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging

            Bryce T. Bajar and Michael Z. Lin

 

4. A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging

            Takahiro Kuchimaru and Shinae Kizaka-Kondoh

 

5. A New Lineage of Artificial Luciferases for Mammalian Cell Imaging

            Sung-Bae Kim and Rika Fujii

 

6. Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems

            Jittima Phonbuppha, Ruchanok Tinikul, and Pimchai Chaiyen

 

Part II: Imaging with Activatable Bioluminescent Probes

 

7. Quantitative Determination and Imaging of Gα_q Signaling in Live Cells via Split-Luciferase Complementation

            Timo Littmann, Takeaki Ozawa, and Günther Bernhardt

 

8. A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules

            Qiaojing Li, Hideaki Yoshimura, and Takeaki Ozawa

 

9. Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution

            Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, and Tetsuya Kitaguchi

 

Part III: Imaging with Functional Substrates and Luciferases

 

10. Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette

            Masahiro Abe, Ryo Nishihara, Sung Bae Kim, and Koji Suzuki

 

11. Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells

            Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Koji Suzuki, and Sung-Bae Kim

 

12. Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays

            Ryo Nishihara, Masahiro Abe, Koji Suzuki, and Sung-Bae Kim

 

Part IV: Imaging with Organic Fluorescent Probes

 

13. Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe

            Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, and Takashi Suzuki

 

14. Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe

            Mitsuyasu Kawaguchi and Hidehiko Nakagawa

 

15. Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Derived Peptide

            Hiroshi Inaba and Kazunori Matsuura

 

16. Discovery of the Environment-Sensitive Near-Infrared (NIR) Fluorogenic Ligand for α₁-Adrenergic Receptors Imaging In Vivo

            Zhao Ma, Lupei Du, and Minyong Li

 

17. Rapid and Sensitive Detection of Cancer Cells with Activatable Fluorescent Probes for Enzyme Activity

            Kyohhei Fujita, Mako Kamiya, and Yasuteru Urano

 

18. Detection of Intracellular Reactive Oxidative Species Using the Fluorescent Probe Hydroxyphenyl Fluorescein

            Wataru Sugimoto, Daisuke Miyoshi, and Keiko Kawauchi

 

19. Development of Near-Infrared Fluorescent Mg²⁺ Probe and Application to Multi-Color Imaging of Intracellular Signals

            Yutaka Shindo, Yuma Ikeda, Yuki Hiruta, Daniel Citterio, and Kotaro Oka

 

20. Long-Term Mg²⁺ Imaging in Live Cells with a Targetable Fluorescent Probe

            Priya Ranjan Sahoo, Toshiyuki Kowada, and Shin Mizukami

 

Part V: Imaging with BRET Probes

 

21. Highly Bright NIR-BRET System for Imaging Molecular Events in Live Cells

            Ryo Nishihara, Koji Suzuki, Sung-Bae Kim, and Ramasamy Paulmurugan

 

22. Ligand-Activatable BRET9 Probes for Imaging Molecular Events in Living Mammalian Cells

            Sung-Bae Kim, Rika Fujii, and Ramasamy Paulmurugan

 

23. Manipulation of Actin Cytoskeleton by Intracellular-Targeted ROS Generation

            Tetsuya Ishimoto and Hisashi Mori

 

24. Bioluminescence Imaging of Neuronal Network Dynamics Using Aequorin-Based Calcium Sensors

            Sandrine Picaud, Bertrand Lambolez, and Ludovic Tricoire

 

25. Method for Detecting Emission Spectral Change of Bioluminescent Ratiometric Indicators via a Smartphone

            Mitsuru Hattori, Tomoki Matsuda, and Takeharu Nagai

 

26. Bioluminescence Resonance Energy Transfer (BRET) Imaging in Living Cells: Image Acquisition and Quantification

            Hiroyuki Kobayashi and Michel Bouvier

 

Part VI: Imaging with FRET Probes

 

27. GPCR Signaling Regulation in Dictyostelium Chemotaxis

            Yoichiro Kamimura and Masahiro Ueda

 

28. Live Cell Imaging Technique to Visualize DAMPs Release during Regulated Cell Death

            Mai Yamagishi and Yoshitaka Shirasaki

 

29. Time-Lapse Imaging of Necroptosis and DAMP Release at Single Cell Resolution

            Shin Murai, Yoshitaka Shirasaki, and Hiroyasu Nakano

 

Part VII: Imaging with Advanced Instrumentation

 

30. High-Throughput Whole Plate Imaging of Cells for Multiple Biological Applications

            Uday Kumar Sukumar, Frezghi Habte, Tarik F. Massoud, and Ramasamy Paulmurugan

 

31. Pinhole Closure Improves Spatial Resolution in Confocal Scanning Microscopy

            Akira Kitamura

 

32. Total Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR

            Masataka Yanagawa and Yasushi Sako

Blurb

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.