Liu's Principles and Practice of Laboratory Mouse Operations: A Surgical Atlas

ISBN-13: 9783030745004 / Angielski / Twarda / 2023 / 1584 str.

Pengxuan Liu; Don Liu

Spis treści

Chapter 1. The Basics

                1.1 Pre-operatively

                1.2 Commonly used instruments

                1.3 Commonly used regional exposure

Chapter 2. Collecting Specimen and Harvesting Tissues

                2.1 Basic Principles of specimen collection

                2.2 Head and neck

                2.3 Organs and other specimens

                2.4 Skinning and tail-ripping techniques

                2.5 Collecting blood

                2.6 Collecting body fluid

                2.7 Imaging, specimen collection

Chapter 3. Drugs Administration

                3.1 Gavage

                3.2 Intraperitoneal injection

                3.3 INtramusclar injection

                3.4 Percutaneous administration

                3.5 Percutaneous injection in subcutaneous gland

                3.6 Intravenous injection

                3.7 Membrane drug administration

                3.8 Organs injection

                3.9 Perfusion

Chapter 4. Surgical Principles

                4.1 Preoperative preparations

                4.2 Prevention tremor

                4.3 Suturing technique 

                4.4 Adhesion

                4.5 Clamping

                4.6 Cutting open or incising

                4.7 Bite

                4.8 Skin excision

                4.9 Surgical punch and electro-cautery

                4.10 Strangulation technique

                4.11 Electro-cautery technique

                4.12 Section cutting

                4.13 Precision truncation of the tail

                4.14 Intubation of the anterior chamber of eye

                4.15 Tracheotomy

                4.16 Oro-tracheal intubation

                4.17 Intestinal intubation

                4.18 Common bile duct intubation

Chapter 5. Vascular Surgery

                5.1 Introduction to Vascular Surgery

                5.2 Bleeding and coagulation

                5.3 Stop Blood Flow

                5.4 Fenestration on blood vessels

                5.5 Intubation

O autorze

Dr. Pengxuan Liu, Ophthalmologist and Manager of Animal Study in the Gene Therapy Lab at the University of Southern California and Scientist at Bayer HealthCare. 

Dr. Don Liu, Professor of Ophthalmology and Director of Ophthalmic Plastic and Orbital Reconstructive Surgery at the University of Missouri, formerly a faculty at the University of Southern California in Los Angelas and Henry Ford Hospital in Detroit

Blurb

This book fills the current void of academic writings on laboratory mouse operation, giving research scientists, graduate students, and laboratory technicians an authoritative textbook and definitive laboratory companion. It covers mouse anatomy, the handling of the mouse, anesthesia, drug administration, specimen collection, organ harvesting and daily laboratory skills as well as advanced micro-surgery techniques. Its detailed description of mouse anatomy corrects many inaccuracies and misconceptions in the literature. It provides a wealth of basic laboratory skills and numerous advanced surgical techniques. The step-by-step explanations, with extensive photographic images and videos, improve the current understanding and practice of laboratory mouse operations.

This book lays the foundation of laboratory mouse operations by offering a clear understanding of the basic principles, updated anatomic studies, and providing invaluable practical tools. It serves a wide audience, including laboratory animal scientists, pharmaceutical science researchers, graduate students in these fields, micro surgeons, veterinarians, and laboratory technicians.