1. Milestones in the development of liquid chromatography 2. Kinetic theories of liquid chromatography 3. Column technology in liquid chromatography 4. General instrumentation in HPLC 5. Liquid-solid chromatography 6. Reversed-phase liquid chromatography 7. Secondary chemical equilibria in reversed-phase liquid chromatography 8. Ultra-high performance and Ultrafast liquid chromatography 9. Nano-liquid chromatography 10. Hydrophilic interaction liquid chromatography 11. Mobile phase selection in liquid chromatography 12. Co-solvents and mobile phase additives (new chapter) 13. Method development in liquid chromatography 14. Physicochemical measurements 15. Theory and practice of gradient elution liquid chromatography 16. Fundamentals of Enantioselective LC (Chiral recognition mechanisms) 17. Hydrophobic interaction chromatography 18. Ion chromatography 19. Size-exclusion chromatography 20. Interaction polymer chromatography 21. Affinity chromatography 22. Multidimensional Liquid Chromatography 23. Process concepts in preparative chromatography 24. Modeling of preparative liquid chromatography 25. Capillary electrochromatography 26. Liquid Chromatography on microchip 27. Mass spectrometric detection, instrumentation and ionization methods 28. Identification and quantitation in Mass Spectrometry 29. Advanced IR and Raman detectors for identification and quantification 30. Advanced spectroscopic detectors for identification and quantification: Nuclear magnetic resonance 31. Prediction of retention in Liquid Chromatography 32. Validation of liquid chromatographic methods - chemometrics and calibration