1. Sample preparation for liquid chromatography 2. Derivatization in liquid chromatography 3. Basic principles for the selection of liquid chromatographic mode for specific application (new chapter) 4. Amino acid and bioamine separations 5. Separation of lipids 6. Analysis of neurotransmitters and their metabolites by liquid chromatography 7. Analysis of natural toxins by liquid chromatography 8. Peptides and Proteins separations 9. Proteomics (new chapter) 10. Liquid chromatographic separation of oligonucleotides 11. Separation of glycans and monosaccharides 12. Metabolic phenotyping (metabonomics/metabolomics) by liquid chromatography-mass spectrometry 13. Principles, current applications, and future perspectives of liquid chromatography-mass spectrometry in clinical chemistry 14. Doping Analysis (new chapter) 15. Environmental analysis: Atmospheric samples 16. Environmental analysis: Emerging pollutants 17. Analysis of Microplastics (new chapter) 18. Environmental analysis: Persistent organic pollutants 19. Foodomics: LC and LC-MS-based omics strategies in food science and nutrition 20. Compositional analysis of foods 21. Multiresidue methods for pesticides and related contaminants in food 22. Analysis of vitamins by liquid chromatography 23. Determination of veterinary drug residues in foods by liquid chromatography-mass spectrometry: Basic and cutting-edge applications 24. Liquid chromatographic separation of enantiomers 25. Liquid chromatography in the pharmaceutical industry 26. Analysis of pharmaceutically and biologically relevant isoforms (new chapter) 27. Forensic toxicology 28. Analysis of Natural Products by liquid chromatography 29. Speciation and element specific detection