Liposomes: Methods and Protocols

ISBN-13: 9781071629536 / Angielski / Twarda / 2023 / 351 str.

Gerard G. M. D'Souza; Hongwei Zhang

Opis

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution.  Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

Spis treści

1. From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First 

Volkmar Weissig 

 

2 Preparation of DRV liposomes

Sophia G. Antimisiaris

 

3 Preparation of small unilamellar vesicles using detergent dialysis method 

Qingyue Zhong and Hongwei Zhang 

 

4 Thin-film hydration followed by extrusion method for liposome preparation 

Hongwei Zhang 

 

5 Ethanol injection method for liposome preparation 

Guangsheng Du and Xun Sun

 

6 Preparation of giant vesicles with mixed single-tail and double-tail lipids 

Lauren A Lowe and Anna Wang 

 

7 Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing 

Jung Yeon Han, Zhu Chen, and Don L. DeVoe

 

8 Preparation of doxorubicin liposomes by remote loading method

Jian Chen

 

9 Magnetic thermosensitive liposomes loaded with Doxorubicin 

Mohamad Alawak, Alice Abu Dayyih, Ibrahim Awak, Bernd Gutberlet, Konrad Engelhardt, and Udo Bakowsky

 

10 Preparation and physical characterization of DNA binding cationic liposomes

Vaibhav Saxena

 

11 Tunable pH sensitive lipoplexes

Helene Dhotel, Michel Bessodes and Nathalie Mignet

 

12 Solid Lipid Nanoparticles for Drug Delivery 

Wei-Chung Luo and Xiuling Lu 

 

13 Stable Discoidal Bicelles – Formulation, Characterization and Functions  

Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh, Justin M. Fang, and Mu-Ping Nieh

 

14 The post-insertion method for the preparation of PEGylated liposomes

Sherif E. Emam, Nehal E. Elsadek, Taro Shimizua, and Tatsuhiro Ishidaa

 

15 Click chemistry for liposome surface modification 

Maria Vittoria Spanedda, Marcella De Giorgi, Béatrice Heurtault, Antoine Kichler, Line Bourel-Bonnet and Benoît Frisch

 

16 Surface Modification of Liposomes Using Folic Acid 

Mengran Guo, Zhongshan He, Xi He and Xiangrong Song

 

17 Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes)

Yuhong Xu

 

18 Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure 

Yanzhi Liu, Zhenshan Jia, Luoyang Ma, and Dong Wang

 

19 Method of simultaneous analysis of liposome components using HPTLC/FID

Sophia Hatziantoniou and Costas Demetzos

 

20 HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes 

Yujie Shi and Xiaona Li

 

21 DPH probe method for liposome-membrane fluidity determination 

Wei He

 

22 Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy 

Anand S. Ubhe 

 

23 Visualization and characterization of liposomes by Atomic Force Microscopy (AFM) 

Konrad Engelhardt, Eduard Preis and Udo Bakowsky

 

24 Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy 

Melani A. Solomon 

 

25 Liposome biodistribution via europium complexes

Nathalie Mignet and Daniel Scherman

 

26 Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome’s uptake by human placenta 

Louise Fliedel, Nathalie Mignet, Thierry Fournier, Karine Andrieux^, and Khair Alhareth