Life at the Nanoscale: Atomic Force Microscopy of Live Cells

ISBN-13: 9789814267960 / Angielski / Twarda / 2011 / 460 str.

Yves F. Dufrene

Opis

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Recenzje

"Atomic force microscopy (AFM) began as a topographical surface imaging technique, but its ability to measure the tiny forces experienced and exerted by cells and the molecules therein has made it an indispensible method for biological research. These forces are central to cell function, and no optical microscopy technique yet surpasses AFM methods for investigating these physical processes at the level of molecules and cells. Now, in this book devoted to the use of AFM in biology, leading scientists using these methods in their own research discuss the great variety of biological applications that have been developed and the many unique discoveries they have allowed. Accessible but informative descriptions and stunning images offer biologists an excellent introduction to this powerful but underexploited methodology. Any biologist considering the use of AFM in their own research is certain to find inspiration in this unique resource."
—Dr. Daniel Evanko, Chief Editor, Nature Methods

"Life at the Nanoscale　provides a state-of-the-art overview about how atomic force microscopy (AFM) can be used to image living cells, to probe their attachment to substrates or to other cells, to measure their mechanical properties, and to assess the forces in single-molecule interactions. Experts in the field have contributed chapters that summarize their hands-on experience in concise, and hence most useful, reviews. So many insightful experiments have been achieved over the last decade, and many of them are now presented in this resourceful book, which comes timely for students and experts who have an interest in the interdisciplinary field of cell biology and in the application of tools that address single molecules."
—Prof. Andreas Engel, Case Western Reserve University, USA

Spis treści

Observing the Nanoscale Organization of Model Biological Membranes by Atomic Force Microscopy. High-Resolution Atomic Force Microscopy of Native Membranes. Microbial Cell Imaging Using Atomic Force Microscopy. Resolving the High-Resolution Architecture, Assembly and Functional Repertoire of Bacterial Systems by Atomic Force Microscopy. Understanding Cell Secretion and Membrane Fusion Processes on the Nanoscale Using the Atomic Force Microscope. Nanophysiology of Cells, Channels and Nuclear Pores. Topography and Recognition Imaging of Cells. High-Speed Atomic Force Microscopy for Dynamic Biological Imaging. Near-Field Scanning Optical Microscopy of Biological Membranes. Quantifying Cell Adhesion Using Single-Cell Force Spectroscopy. Probing Cellular Adhesion at the Single-Molecule Level. Mapping Membrane Proteins on Living Cells Using the Atomic Force Microscope. Probing Bacterial Adhesion Using Force Spectroscopy. Force Spectroscopy of Mineral-Microbe Bonds. Single-Molecule Force Spectroscopy of Microbial Cell Envelope Proteins. Probing the Nanomechanical Properties of Viruses, Cells and Cellular Structures. Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements. Investigating Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force Microscopy. The Role of Atomic Force Microscopy in Advancing Diatom Research into the Nanotechnology Era. Atomic Force Microscopy for Medicine.