Leukemia Stem Cells: Methods and Protocols

ISBN-13: 9781071608098 / Angielski / Twarda / 2020 / 479 str.

César Cobaleda; Isidro Sánchez-García

Spis treści

Part I: Introductory Reviews

 

1. Introduction and Classification of Leukemias

            Geoffrey Brown

 

2. Leukemia Stem Cells: Concept and Implications

            Isidro Sánchez-García and César Cobaleda

 

3. Leukemia Stem Cell Drug Discovery

            César Cobaleda and Isidro Sánchez-García

 

Part II: Protocols

 

4. Analysis and Isolation of Mouse Leukemic Stem Cells

            Fang Dong, Haitao Bai, and Hideo Ema

 

5. Mass Cytometry of Hematopoietic Cells

            Astraea Jager, Jolanda Sarno, and Kara Davis

 

6. PCR Technology to Identify Minimal Residual Disease

            Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group

 

7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies

            Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Brüggemann, on behalf of the EuroClonality-NGS Working Group

 

8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

            Daniel Hein, Arndt Borkhardt, and Ute Fischer

 

9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells

            Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic

 

10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

            Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly

 

11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation

            Kyomi J. Igarashi and Ryo Yamamoto

 

12. Experimental Competitive Bone Marrow Transplant Assays

            Roxann Hétu-Arbour, Sarah Bouali, and Krista M. Heinonen

 

13. Human T-ALL Xenografts

            Patricia Fuentes, María L. Toribio, and Sara González-García

 

14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts

            Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci

 

15. Leukemic Stem Cell Culture in Cytokine-Free Medium

            Xiaolei Liu and Peter S. Klein

 

16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

            Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman

 

17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

            Cristina Prieto, Damia Romero-Moya, and Rosa Montes

 

18. Lentiviral Transduction for Optimal LSC/HSC Manipulation

            Gustavo Mostoslavsky

 

19. Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes

            Yu Wei Zhang, Julian Mess, and Nina Cabezas-Wallscheid

 

20. Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes

            Sabrina Jacobs, Leonid V. Bystrykh, and Mirjam E. Belderbos

 

21. Arrayed Molecular Barcoding of Leukemic Stem Cells

            Marion Chapellier and Marcus Järås

 

22. In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis

            Carolina Vicente-Dueñas

 

23. In Situ Hematopoietic Stem Cell Imaging

            Aparna Venkatraman, Sarah E. Smith, Sandra Pinho, Meng Zhao, Linheng Li, and Paul Frenette

 

24. A Genome-Editing System for Therapeutical Targeting of Stem Cells

            Giacomo Frati and Annarita Miccio

 

25. Method for the Generation of Induced Hematopoietic Stem Cells

            Leonid Olender, Klil Levy, and Roi Gazit

 

26. Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells

            André G. Deslauriers, Andriana G. Kotini, and Eirini P. Papapetrou

 

27. High Content Imaging to Phenotype Human Primary and iPSC-Derived Cells

            Lorenzo Veschini, Heba Sailem, Disha Malani, Vilja Pietiäinen, Ana Stojiljkovic, Erika Wiseman, and Davide Danovi

 

28. Bioinformatic Methods to Identify Mutational Signatures in Cancer

            S.M. Ashiqul Islam and Ludmil B. Alexandrov

Blurb

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.