Lentivirus Gene Engineering Protocols

ISBN-13: 9781607615323 / Angielski / Twarda / 2009 / 228 str.

Maurizio Federico

Opis

Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study."

Spis treści

Part I: Overview 1. Lentiviral Vector-Mediated Gene Transfer and RNA Silencing Technology in Neuronal Dysfunctions Jean-Luc Dreyer Part II: Advances in Lentiviral Vector Technology 2. New Protocol for Lentiviral Vector Mass Production María Mercedes Segura, Alain Garnier, Yves Durocher, Sven Ansorge, and Amine Kamen 3. Flexibility in Cell Targeting by Pseudotyping Lentiviral Vectors Daniela Bischof and Kenneth Cornetta 4. Doxycycline Regulated Lentiviral Vectors David Markusic and Jurgen Seppen 5. Strategies to Insulate Lentiviral Vector-Expressed Transgenes Ali Ramezani and Robert G. Hawley 6. Integrase Defective, Nonintegrating Lentiviral Vectors Zuleika Michelini, Donatella Negri, and Andrea Cara 7. Lentivirus-Based Virus-Like Particles as a New Protein Delivery Tool Claudia Muratori, Roberta Bona, and Maurizio Federico Part III: New Lentiviral Vector Applications 8. Lentiviral Vector-Mediated Transgenesis in Human Embryonic Stem Cells Zhong-Wei Du and Su-Chun Zhang 9. Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells Mark S.K. Chong and Jerry Chan 10. Manipulating the Cell Differentiation Through Lentiviral Vectors Valeria Coppola, Cesare Galli, Maria Musumeci, and Désirée Bonci 11. Lentiviral Vector Transduction of Dendritic Cells for Novel Vaccine Strategies Lung-Ji Chang Part IV: The Short RNA Technologies Applied by Lentiviral Vectors 12. Lentiviral Vector-Mediated Expression of pre-miRNAs and AntagomiRs Michaela Scherr, Letizia Venturini, and Matthias Eder 13. The RNA Silencing Technology Applied by Lentiviral Vectors inOncology Hidetoshi Sumimoto and Yutaka Kawakami 14. Lentiviral Vector Engineering for Anti-HIV RNAi Gene Therapy Olivier ter Brake, Jan-Tinus Westerink, and Ben Berkhout 15. Lentivirus-Expressed siRNA Vectors Against Alzheimer Disease Kevin A. Peng and Eliezer Masliah