Legionella: Methods and Protocols

ISBN-13: 9781493990474 / Angielski / Twarda / 2019 / 476 str.

Carmen Buchrieser; Hubert Hilbi

Spis treści

Part I: Ecology, Physiology and Genetics of Legionella

1. Analysis of Iron Requirements and Siderophore Production

Denise M. Burnside, and Nicholas P Cianciotto

 

2. The Pathometabolism of Legionella Studied by Isotopologue Profiling

Klaus Heuner, Mareike Kunze, Fan Chen, and Wolfgang Eisenreich

 

3. Quantification of Viable but Non-culturable Cells of Legionella pneumophila

Rosalie S. Braun, Nilmini Mendis, Laam Li, and Sebastien P. Faucher

 

4. Selection of Legionella Virulence-related Traits by Environmental Protozoa

Francisco Amaro and Howard Shuman

 

5. Migration of Acanthamoeba castellanii through Legionella Biofilms

Ramon Hochstrasser and Hubert Hilbi

 

6. Scar-free Genome Editing in Legionella pneumophila

Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, and Christophe Gilbert

 

7. Querying Legionella Genomes using Transposition-sequencing

Léo Hardy and Xavier Charpentier

 

8. Screening Targeted Legionella pneumophila Mutant Libraries in vivo using INSeq

Stephanie R. Shames

 

9. Genetic Manipulation of non-pneumophila Legionella: Protocols Developed for Legionella longbeachae

Elizabeth Korevaar, Chen Ai Khoo, and Hayley J. Newton

 

Part II: Cellular Microbiology of Legionella

10. Quantitative Imaging Flow Cytometry of Legionella-containing Vacuoles in Dually Fluorescence-labelled Dictyostelium

Amanda Welin, Stephen Weber, and Hubert Hilbi

 

11. Sorting of Phagocytic Cells Infected with Legionella pneumophila

Monica Rolando and Carmen Buchrieser

 

12. Single Cell Analysis of Legionella and Legionella-infected Acanthamoeba by Agarose Embedment

Nicolas Personnic, Bianca Striednig, and Hubert Hilbi

 

13. Mitochondrial Dynamics and Activity in Legionella-Infected Cells

Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, and Pedro Escoll

 

14. Perturbation of Legionella Cell Infection by RNA Interference

Bernhard Steiner, A. Leoni Swart, and Hubert Hilbi

 

Part III: Biochemical Assays to Study Legionella Effectors and Enzymes

15. Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila

Tomoko Kubori and Hiroki Nagai

 

16. In situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems using Electron Cryotomography

Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, and Grant J. Jensen

 

17. Methods for Non-canonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins

Jiazhang Qiu and Zhao-Qing Luo

 

18. Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila

Nadezhda Levanova, Irina Tabakova, Thomas Jank,  and Yury Belyi

 

19. Determination of in vivo Interactomes of Dot/Icm type IV Secretion System Effectors by Tandem Affinity Purification

Ernest C. So, Aurélie Mousnier, Gad Frankel_, and Gunnar N. Schroeder

 

20. Inflammasome Activation in Legionella-infected Macrophages

Danielle P. A. Mascarenhas and Dario S. Zamboni

 

Part IV. Immunity and Host Response Against Legionella

21. Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila

Lina Scheithauer and Michael Steinert

 

22. The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence

Gad Frankel and Gunnar N. Schroeder

 

23. Dictyostelium Host Response to Legionella Infection: Strategies and Assays Salvatore Bozzaro, Simona Buracco, Barbara Peracino, and Ludwig Eichinger

 

24. 6.1.3 The Caenorhabditis elegans model of Legionella infection

Ann Karen C. Brassinga and Costi D. Sifri

 

25. The Mouse as a Model for Pulmonary Legionella Infection

Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel^, and

Elizabeth L. Hartland

 

Part V. Genomics, Transcriptomics, and Proteomics of Legionella

26. Targeted Metagenomics of Microbial Diversity in Free-living Amoebae and Water Samples

Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, and Yann Héchard

 

27. Analyses of the Pulmonary Microbiome Composition of Legionella Pneumophila-Infected Patients

Ana Elena Pérez-Cobas and Carmen Buchrieser

28. Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry

Sandra Maaß, Gina Moog, and Dörte Becher

Blurb

The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila.  Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella,  and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.