Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue

ISBN-13: 9781493903801 / Angielski / Twarda / 2014 / 307 str.

Lidia Bakota; Roland Brandt

Opis

Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field. This detailed volume seeks to introduce key questions, to provide detailed information on how to acquire data by laser-scanning microscopy, and to examine how to use the often huge digital data set in an efficient manner to extract maximum information. Thus the book not only provides a compilation of diverse protocols but aims to bring together biological bench work, laser scanning microscopy, and mathematical, computer-assisted data analysis to grasp novel insights of form, dynamics, and interactions of microscopy-sized biological objects. Written in the popular Neuromethods series format, chapters include the kind of practical implementation advice that promises successful results.Wide-ranging and innovative, Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue will stimulate the reader to make efficient use of the application of laser scanning microscopy for his or her own research question.

Spis treści

1. Translation, Touch, and Overlap in Multi-Fluorescence Confocal Laser Scanning Microscopy to Quantitate Synaptic Connectivity

            Floris G. Wouterlood and Jeroen A.M. Beliën

 

2. Surgical Procedures to Study Microglial Motility in the Brain and in the Spinal Cord by In Vivo Two-Photon Laser-Scanning Microscopy

            Alexander Cupido, Bogdan Catalin, Heinz Steffens, and Frank Kirchhoff

 

3. Analysis of Brain Projection Systems Using Third-Generation Neuroanatomical Tracers and Multiple Fluorescence Laser Scanning Microscopy

            Floris G. Wouterlood

 

4. Combining Multi-Channel Confocal Laser Scanning Microscopy with Serial Section Reconstruction to Analyze Large Tissue Volumes at Cellular Resolution

            Federico Luzzati

 

5. Modeling Excitotoxic Ischemic Brain Injury of Cerebellar Purkinje Neurons by Intravital and In Vitro Multi-Photon Laser Scanning Microscopy

            Amanda J. Craig, Gary D. Housley, and Thomas Fath

 

6. Analysis of Morphology and Structural Remodeling of Astrocytes

            Tatjana C. Jakobs

 

7. Quantitative Analysis of Axonal Outgrowth in Mice

            Rosa-Eva Huettl and Andrea B. Huber

 

8. Zebrafish Brain Development Monitored by Long-Term In Vivo Microscopy: A Comparison Between Laser Scanning Confocal and 2-Photon Microscopy

            Nicolas Dross, Carlo Antonio Beretta, Peter Bankhead, Matthias Carl, and Ulrike Engel

 

9. Analysis of Actin Turnover and Spine Dynamics in Hippocampal Slice Cultures

            Kristin Michaelsen-Preusse, Yves Kellner, Martin Korte, and Marta Zagrebelsky

 

10. Quantitative Geometric Three-Dimensional Reconstruction of Neuronal Architecture and Mapping of Labeled Proteins from Confocal Image Stacks

            Jan Felix Evers and Carsten Duch

 

11. Confocal Microscopy Used for the Semiautomatic Quantification of the Changes in Aminoacidergic Fibers During Spinal Cord Regeneration

            Blanca Fernández-López, Antón Barreiro-Iglesias, and María Celina Rodicio

 

12. Reconstruction and Morphometric Analysis of Hippocampal Neurons from Mice Expressing Fluorescent Proteins

            Nataliya Golovyashkina, Frederik Sündermann, Roland Brandt, and Lidia Bakota

 

13. Machine Learning to Evaluate Neuron Density in Brain Sections

            Lorène Penazzi, Frederik Sündermann, Lidia Bakota, and Roland Brandt

 

14. Shearlet-Analysis of Confocal Laser-Scanning Microscopy Images to Extract Morphological Features of Neurons

            Frederik Sündermann, Sebastian Lotter, Wang-Q Lim, Nataliya Golovyashkina, Roland Brandt, and Gitta Kutyniok