Laser Capture Microdissection: Methods and Protocols

ISBN-13: 9781493975570 / Angielski / Twarda / 2018 / 412 str.

Graeme I. Murray

Opis

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies.

Spis treści

1. Laser Capture Microdissection: Insights into Methods and Applications

            Meera Mahalingam

2. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues

            Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw

3. Isolation of DNA and RNA from Laser Microdissected Tissue Samples

            Yelena G. Golubeva and Andrew C. Warner

4. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

            DaRue A. Prieto, Gordon Whitely, Donald J. Johann, Jr., and Josip Blonder

5. Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens

            Lavinia Mägel, Stephan Bartels, and Ulrich Lehmann

6. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model

            Nadia P. Castro and Yelena G. Golubeva

7. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease

            Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella

8. Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue

            Michele Cummings, Georgia Mappa, and Nicolas M. Orsi

9. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions

            Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall

10. Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone

            Efrain Pacheco, Rong Hu, and Scott Taylor

11. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain

            Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag

12. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience

            Renée Morris and Prachi Mehta

13. Laser Capture Microdissection in Traumatic Brain Injury Research:  Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses

            Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich

14. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography – Mass Spectrometry

            Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May

15. Immuno-Guided  Laser Capture Microdissection of Glial Cells for mRNA Analysis

            Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud

16. Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain

            Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath

17. Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR

            Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado

18. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics

            Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski

19. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR

            Johanna Duda, Michael Fauler, Jan Gründemann, and Birgit Liss

20. Laser Capture Microdissection for Layer-Specific Analysis of Enteric Ganglia

            Corinna Rosenbaum, Martina Böttner, Thilo Wedel, and Marco Metzger

21. A Laser Microdissection - Liquid Chromatography - Tandem Mass Spectrometry Workflow for Post-Mortem Analysis of Brain Tissue

            David C. Hondius, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, Ka Wan Li, and August B. Smit

22. Laser Capture Microdissection and RNA Extraction from Perfusion-Fixed Cartilage and Bone Tissue from Mice Implanted with Human iPSC-Derived MSCs in a Calvarial Defect Model

            Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, and Joel S. Pachter

23. Laser Capture Microdissection-Based RNA-Seq of Barley Grain Tissues

            Ronny Brandt, Martin Mascher, and Johannes Thiel

Blurb

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.