Laboratory Methods in Cell Biology: Imaging: Volume 113

ISBN-13: 9780124072398 / Angielski / Twarda / 2013 / 414 str.

P Michael Conn

Opis

Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

• Covers the most important laboratory methods in cell biology
• Chapters written by experts in their fields

Spis treści

1. Single Molecule FRET Microscopy
2. Bioluminescent Imaging of Gene Expressions
3. Confocal Imaging
4. Electron Microscopy of Bone
5. Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments
6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions
7. Histochemistry
8. Live Imaging
9. Click Labeling for Single-Molecule FRET
10. High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers
11. Correlation Microscopy
12. Stereology of the Lung
13. Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies.
14. Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy
15. Cryomicroscopy
16. In situ Hybridization
17. Imaging RNA