LC-MS in Drug Bioanalysis

ISBN-13: 9781489990891 / Angielski / Miękka / 2014 / 472 str.

Q. Alan Xu; Timothy L. Madden

Opis

Clinical pharmacology plays an important role in today's medicine. Due to the high sensitivity, selectivity, and affordability of a mass spectrometer (MS), the high performance liquid chromatography - mass spectrometry (LC-MS) analytical technique is widely used in the determination of drugs in human biological matrixes for clinical pharmacology. Specifically, LC-MS is used to analyze: anticancer drugs antidementia drugs antidepressant drugs antiepileptic drugs antifundal drug antimicrobial drugs antipsychotic drugs antiretroviral drugs anxiolytic/hypnotic drugs cardiac drugs drugs for addiction immunosuppressant drugs mood stabilizer drugs This book will primarily cover the various methods of validation for LC-MS techniques and applications used in modern clinical pharmacology.

Spis treści

Internal standards for quantitative LC-MS bioanalysis.- Method development, validation and sample analysis for regulated quantitative bioanalysis using LC-MS/MS.- Impact of sampling paper/cards on bioanalytical quantitation via dried blood spots by liquid chromatography - mass spectrometry.- Highly sensitive pharmaceutical and clinical analysis using selective solid-phase extraction coupled to microflow liquid chromatography and isotope-dilution mass spectrometry.- Pitfalls of LC-MS/MS in the clinical laboratory.- Therapeutic drug monitoring to support clinical pharmacogenomics.- Liquid chromatography - mass spectrometry for the determination of antidepressants and some of their major metabolites in human biological matrices.- The analysis of antipsychotic drugs in human biosamples by LC-MS.- Therapeutic drug monitoring of targeted anticancer therapy - tyrosine kinase inhibitors and selective oestrogen receptor modulators: a clinical pharmacology laboratory perspective.- Applications of mass spectrometry in analyses of steroid hormones.- Liquid chromatography - mass spectrometric analysis of tropane alkaloids in mammalian samples: techniques and applications.- Analysis of illicit drugs in human biological samples by LC-MSn.- Use of matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) in the development of novel small molecule drugs.- A planar integrated micro mass spectrometer.

Blurb

            High performance liquid chromatography – mass spectrometry (LC-MS) combines the separation power of liquid chromatography with the high sensitivity and selectivity of mass spectrometry. Therefore, LC-MS becomes the method of choice for drug bioanalysis in therapeutic drug monitoring, toxicology, and drug discovery and development. LC-MS in Drug Bioanalysis offers discussions on:

            ● LC-MS/MS method development and validation in a regulated environment

            ● Internal standard use in LC-MS/MS bioanalysis

            ● Pitfalls of LC-MS/MS and how to overcome them

            ● Dried blood spot sampling

            ● Microflow LC-MS/MS

            ● LC-MS/MS in therapeutic drug monitoring

            ● Applications of LC-MS/MS in drug bioanalysis

This book will aid readers to improve their skills in LC-MS/MS method development, validation, and applications in the analysis of drugs and their metabolites in complex biological matrices.  In addition, this book provides detailed information on the bioanalysis of antidepressants, antipsychotics, steroid hormones, anticancer agents, and drugs of abuse. Newer methodologies are discussed including the use of unique sample preparation techniques and the implementation of imaging mass spectrometry in the localization of drug and drug metabolites in tissues.