LC-MS in Drug Analysis: Methods and Protocols

ISBN-13: 9781493961993 / Angielski / Miękka / 2016 / 224 str.

Loralie J. Langman; Christine L. H. Snozek

Opis

This Methods in Molecular Biology(TM) volume covers LC-MS screening for drugs of abuse, as well as targeted measurement of analytes including abused drugs, toxic compounds, and therapeutic agents. Includes step-by-step protocols, tips and troubleshooting notes.

Recenzje

From the book reviews:

"This is a compilation of LC-MS/MS technical procedures for drug analysis. ... It is written for practicing toxicologists or clinical chemists whose daily practice includes analysis for these clinically important substances. It also would be of interest to anyone curious about clinical laboratory testing for any of these compounds or interested in clinical applications of LC-MS/MS. ... You will need this book if you are involved in LC-MS/MS analysis for compounds of clinical interest." (Valerie L. Ng, Doody's Book Reviews, November, 2012)

Spis treści

1. An Introduction to Drug Testing – The Expanding Role of Mass Spectrometry

            Catherine Hammett-Stabler and Steven W. Cotton

 

2. LC-MS vs. GC-MS, Online Extraction Systems, Advantages of Technology for Drug Screening Assays

            Pierre Marquet

 

3. LC-MS/MS Techniques for High Volume Screening of Drugs of Abuse and Target Drug Quantitation in Urine/Blood Matrices

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

 

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

 

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

 

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

 

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

 

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

 

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

 

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

 

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

 

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

 

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

 

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

 

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

 

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

 

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

 

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

 

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

 

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

 

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

 

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

 

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

 

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

 

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

 

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

 

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

 

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

 

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

 

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

 

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

 

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

 

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

 

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

 

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

 

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

 

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

 

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

 

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

4. Benzodiazepines and Metabolites from Biological Fluids by Liquid Chromatography Electrospray Tandem Mass Spectrometry

            Cynthia L. Morris-Kukoski, Jason E. Schaff, and Louis J. Reda

 

5. Opiate Screening and Quantitation in Urine/Blood Matrices using LC-MS/MS Techniques

            Jeff C. Eichhorst, Michele L. Etter, Patricia L. Hall, and Denis C. Lehotay

 

6. Synthetic Opioid Analysis by LC-MS/MS

            David M. Garby and Lynn A. Cheryk

 

7. The Determination of Cannabinoids Using Liquid Chromatography with Mass Spectrometric Detection

            Oscar Quintela and Dennis J. Crouch

 

8. Cocaine and Metabolites by LC-MS/MS

            Christine L. H. Snozek, Matthew W. Bjergum, and Loralie J. Langman

 

9. Quantitation of Amphetamine Type Stimulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

10. Detection of Prohibited Substances by Liquid Chromatography Tandem Mass Spectrometry for Sports Doping Control

            Brian D. Ahrens, Borislav Starcevic, and Anthony W. Butch

 

11. LC-MS/MS Screen for Xenobiotics and Metabolites

            François-Ludovic Sauvage and Pierre Marquet

 

12. Qualitative Identification of Rodenticide Anticoagulants by LC-MS/MS

            Robert A. Middleberg and Joseph Homan

 

13. Hypoglycemic Agent Screening by LC-MS/MS

            Eric W. Korman, Loralie J. Langman, and Christine L. H. Snozek

 

14. LC-MS/MS Method for the Detection of Common Laxatives

            Robert A. Middleberg and Joseph Homan

 

15. Simultaneous Determination of Cyclosporine, Sirolimus and Tacrolimus in Whole Blood Using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS)

            Uttam Garg, Ada Munar, and C. Clinton Frazee III

 

16. Quantification of Tricyclic Antidepressants Using UPLC-MS/MS

            Kamisha L. Johnson-Davis, JoEtta M. Juenke, Rebecka Davis, and Gwendolyn A. McMillin

 

17. Quantitation of First- and Second-Generation Antipsychotics by LC-MS/MS

            Elizabeth R. Kiely and Heather M. Antonides

 

18. Analysis of Selected Anticonvulsants by High Performance Liquid Chromatography-Tandem Mass Spectrometry

            Jennifer A. Collins and Gregory C. Janis

 

19. Therapeutic Drug Monitoring of Tamoxifen Using LC-MS/MS

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

            Simone M. Tchu, Kara L. Lynch, and Alan H. B. Wu

Blurb

Liquid-Chromatography-Mass-Spectrometry procedures have been shown to be successful when applied to drug development and analysis. LC-MS in Drug Analysis: Methods and Protocols provides detailed LC-MS/MS procedures for the analysis of several compounds of clinical significance.  The first chapters provide the reader with an overview of mass spectroscopy, its place in clinical practice, its application of MS to TDM and toxicology, and the merits of LC-MS(/MS) and new sample preparation techniques.  The following chapters discuss different approaches to screening for drugs of abuse and for general unknowns, as well as targeted measurement of specific analytes or classes of analytes including abused drugs, toxic compounds, and therapeutic agents.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, LC-MS in Drug Analysis: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.