Kidney Research: Experimental Protocols

ISBN-13: 9781493933518 / Angielski / Twarda / 2015 / 292 str.

Tim D. Hewitson; Edward R. Smith; Stephen G. Holt

Opis

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification.

Spis treści

Part I: In Vitro, In Vivo and Ex Vivo Models

1. Isolation and Primary Culture of Murine Podocytes with Proven Origin
Bart Smeets, Nazanin Kabgani, and Marcus J. Moeller

 

2. Propagation and Culture of Human Renal Fibroblasts
Sven-Jean Tan and Tim D. Hewitson

 

3. Isolation and Propagation of Rat Peritoneal Mesothelial Cells
Qin Zhou and Xueqing Yu

 

4. Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications
Masashi Mizuno and Yasuhiko Ito

5. Renal Sympathetic Denervation in Rats
Masahiro Eriguchi and Kazuhiko Tsuruya

6. Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds
Mei Jin, Yu Yaling, Wang Zhibing, and Zhang Jianse

 

Part II: Imaging Techniques

7. Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
Kevin M. Bennett, Scott C. Beeman, Edwin J. Baldelomar, Min Zhang, Teresa Wu, Bradley D. Hann, John F. Bertram, and Jennifer R. Charlton

 

8. Biopsychronology: A Method Using Live Tissue Staining to Imag

e Cell Function in the Kidney
Muhammad Imtiaz Ashraf, Dietmar Fries, Werner Streif, Felix Aigner, Paul Hengster, Jakob Troppmair, and Martin Hermann

Part III: In Vivo Analysis of Metabolism in Ischemia-Reperfusion

9. Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats
Maarten P. Koeners, Connie P. C. Ow, David M. Russell, Roger G. Evans, and Simon C. Malpas

 

10. Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury
Andreas Pohlmann, Karen Arakelyan, Erdmann Seeliger, and Thoralf Niendorf

 

11. Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological  Measurements
Kathleen Cantow, Karen Arakelyan, Erdmann Seeliger, Thoralf Niendorf, Andreas Pohlmann

 

12. Intavital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
David M. Small, Washington Y. Sanchez, and Glenda C. Gobe

Part IV: Vascular Mineralization

13. Vascular Calcification in Uraemia: New-Age Concepts about an Old-Age Problem
Edward R. Smith

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14. An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
Kristen J. Kelynack and Stephen G. Holt

 

15. The Isolation and Quantitation of Fetuin-A Containing Calciprotein Particles from Biological Fluids
Edward R. Smith

 

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6. Combining Near Infrared Fluorescent Imaging for Calcification and Inflammation in Vascular Tissue Samples Ex Vivo
Matthias Derwall

 

Part V: Analytical Techniques

17. Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR
Tim D. Hewitson, Michael Christie, and Edward. R. Smith

 

18. Using Phos-Tag in Western Blotting Analysis to Evaluate Protein Phosphorylation

Takahiro Horinouchi, Koji Terada, Tsunehito Higashi, and Soichi Miwa

19. Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections

Katherine Ververis, Selly Marzully, Chrishan S. Samuel, Tim D. Hewitson, and Tom Karagiannis

Blurb

This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.