Ionotropic Glutamate Receptor Technologies

ISBN-13: 9781493948956 / Angielski / Miękka / 2016 / 304 str.

Gabriela K. Popescu

Spis treści

Part I: Protein Assembly and Trafficking

1. Assaying AMPA Receptor Oligomerization

Catherine L. Salussolia, Quan Gan, and Lonnie P. Wollmuth

2. A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells

Mark R. P. Aurousseau, Hugo McGuire, Rikard Blunck, and Derek Bowie

 3. Counting NMDA Receptors at the Cell Surface

Martin Horak and Young Ho Suh

4. Electron Microscopy Analysis of AMPA receptors in Dendritic Spines

Audra A. Kramer, Amber N. Petersen, and Nashaat Z. Gerges

5. Electrophysiological Tagging of Ionotropic Receptors

Andres Barria

6. Functional Detection of Novel Triheteromeric NMDA Receptors

Sanjay S. Kumar

Part II:  Protein Structure and Dynamics

7. Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses

Kelly A. Krogh and Stanley A. Thayer

8. NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains

Christopher P. Ptak, Ahmed H. Ahmed, and Robert E. Oswald

9. Computing Conformational Free Energies of iGluR Ligand-Binding Domains

Alvin Yu, Tyler Wied, John Belcher, and Albert Y. Lau

10. LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors

Rita E. Sirrieh and Vasanthi Jayaraman

11. Assaying the Energetics of NMDA Receptor Pore Opening

Rashek Kazi, Melissa Daniel, and Lonnie P. Wollmuth

 

 

Part III: Protein Function

12. Constructing a Rapid Solution Exchange System

David M. MacLean

13. Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics

Mark W. Fleck

14. Analysis of Whole-Cell NMDA Receptor Responses

Vojtech Vyklicky, Miloslav Korinek, Ales Balik, Tereza Smejkalova, Barbora Krausova, and Ladislav Vyklicky

15. Expression, Purification, and Crystallization of Full Length

Ionotropic Glutamate Receptors

Maria V. Yelshanskaya, Kei Saotome, Minfen Li, and Alexander I. Sobolevsky

16. Timing AMPA Receptor Activation with Laser-pulse Photolysis

Li Niu

17. Current Recording and Kinetic Analyses for Single AMPA Receptors

Kinning Poon, Robert E. Oswald, and Linda M. Nowak

18. Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings

Kirstie A. Cummings, Gary J. Iacobucci, Gabriela K. Popescu

Blurb

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function, and physiology.  Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition, and subcellular location of iGluRs;   approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

 Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.