Ion and Molecule Transport in Lysosomes

ISBN-13: 9781138560390 / Angielski / Twarda / 2020 / 239 str.

Bruno Gasnier; Michael X. Zhu

Opis

This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.

Spis treści

Preface. Editors. Contributors. Endosomal and Lysosomal Electrophysiology. Chloride Transport across the Lysosomal Membrane. Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities. TRPML Subfamily of Endolysosomal Channels: Concepts and Methods. Investigating the Role of Two-Pore Channel 2. (TPC2) in Zebrafish Neuromuscular Development. Functional Study of Lysosomal Nutrient Transporters. Lysosomal Vitamin B12. Trafficking. Detection of Lysosomal Membrane Permeabilization. Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag. A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation. Index

O autorze

Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes. Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.