Invariant Natural Killer T-Cells: Methods and Protocols

ISBN-13: 9781071617779 / Angielski / Miękka / 2022

Liu, Chaohong

Opis

This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.

Spis treści

1. Genetic Analysis of iNKT Cell Development and Function

            Julie Sahler, Orchi Anannya, Candice Limper, Brian Imbiakha, Tim Pierpont, and Avery August

 

2. Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells

            Dapeng Zhou, Chengfeng Xia, Peng G. Wang, Zhiyuan Li, Wenpeng Zhang, Guanghui Ni, and Jiaxi Cheng

 

3. Retroviral Transduction of NKT Hybridoma Cells

            Ke Wang, Rong Jin, and Qing Ge

 

4. Methods for Studying Mouse and Human Invariant Natural Killer T-Cells

            Yang Zhou, Yan-Ruide Li, Samuel Zeng, and Lili Yang

 

5. Isolation and Detection of Murine iNKT Cells in Different Organs

            Mengqing Cong, Xiang Li, Haopeng Fang, Li Bai, Xucai Zheng, and Bofeng Li

 

6. Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

            Wenjing He, Dongmei Ye, and Yifang Gao

 

7. Isolation and Characterization Methods of Human Invariant NKT Cells

            Rui Liu and Hua Wang

 

8. Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry

            Vibhuti Joshi and Masaki Terabe

 

9. Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry

            Jihene Klibi and Kamel Benlagha

 

10. iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach

            Marcella Cipelli, Theresa Ramalho, Cristhiane Favero de Aguiar, and Niels Olsen Saraiva Camara

 

11. Expansion of Human iNKT Cells Ex Vivo

            Jing Wang, Chen Zhao, and Jianqing Xu

 

12. The Expansion and Cytotoxicity Detection of Human iNKT Cells

            Xue Cheng, Xiaosheng Tan, Rui Dou, Xiongwen Wu, and Xiufang Weng

 

13. Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

            Natasha K. Khatwani, Kelly J. Andrews, and Asha B. Pillai

 

14. Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice

            Zhou Hong, Zeng Zhutian, and Wang Fei

 

15. In Vivo Cytotoxicity by α-GalCer-Transactivated NK Cells

            Patrick T. Rudak and S.M. Mansour Haeryfar

 

16. Redirecting iNKT Cell Anti-Tumor Immunity with αGalCer/CD1d-scFv Fusion Proteins

            Lianjun Zhang and Alena Donda

 

17. Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development

            Jana L. Raynor and Hongbo Chi