Intrabodies: Basic Research and Clinical Gene Therapy Applications

ISBN-13: 9783662121214 / Angielski / Miękka / 2014 / 211 str.

Wayne A. Marasco

Opis

Antibodies have long been used in the biomedical sciences as in vitro tools for the identification, purification and functional manipulation of target antigens. They are also being exploited in vivo for diagnostic and therapeutic applications and play an important role in cancer and AIDS research. This book summarizes preclinical studies from laboratories worldwide that have used intrabodies - intracellular antibodies - for gene therapy and research applications.

Spis treści

1. Designing Intrabodies: Structural Features and the Use of Intracellular Trafficking Signals.- 2. Phage Libraries for Generation of Single Chain Fv Antibodies for Intracellular Immunization.- 3. Phenotypic Knockout of the Human Interleukin-2 Receptor ? Chain On Primary and HTLV-I Transformed T Cells.- 4. Intracellular Antibodies as Tools to Study ErbB Receptor Tyrosine Kinases.- 5. Neuroantibodies: The Use of Recombinant Antibody Expression in the Central Nervous System.- 6. Intracellular Antibody-Mediated Knockout of the ErbB-2 Oncoprotein as a Cancer Gene Therapy Approach.- 7. Intracellular Targeting of Oncogenes: A Novel Approach for Cancer Therapy.- 8. Intrabodies Against the HIV-1 Regulatory Proteins: Tat and Rev as Targets for Gene Therapy.- 9. Gene Therapy for HIV-1 Using Intracellular Antibodies Against HIV-1 Gag Proteins.- 10. Single Chain Variable Fragment-Based Strategies for Anti-HIV-1 Gene Therapy: Targeting the Viral Preintegration Complex and Combination Molecular Approaches.