Inhibitors Tools in Cell Research: 20. Colloquium Am 14.-16. April 1969

ISBN-13: 9783540044413 / Angielski / Miękka / 1969 / 416 str.

Theodor Ba1/4cher

Opis

The scope of the 20th Mosbach Colloquium may be best illustrated by the following notes sent to the speakers when the colloquium was organized. "1) The application of inhibitors in cell biology has led to decisive insight into the organisation of cellular units. The subject should be treated against a background of current aspects of cell biology. In some areas of research, a fairly complete picture of the functions and cooperative interactions of the units has already emerged. We will discuss mainly these areas. 2) At this colloquium we want to contribute illustrations of the useful application of inhibitors to biological problems. Due to limited knowledge, inhibitors are sometimes incorrectly employed. This applies both to the planning of investigations and to the con. clusions drawn ("use of inhibitors by uninhibited workers"). 3) Inhibitors themselves are interesting substances and their mechanisms of action constitute fascinating problems." The colloquium has been subdivided into five sections. The known chemical structures of the inhibitors discussed are given in an Appendix. We gratefully appreciate the cooperation of the speakers. To a great extent, they managed the coordination of contributions to each of the five subdivisions. We regret that no contributors from Eastern Europe were able to participate."

Spis treści

I. DNA-Dependent Processes.- DNA-Replication in vivo and in vitro.- Discussion.- Inhibitors Acting on DNA and their Use to Study DNA Replication and Repair.- Structure and Function of DNA-dependent RNA-Polymerase.- Inhibitors of the RNA Polymerase Reaction.- Interaction of RNA Polymerase with Rifamycin.- Effects of some RNA Polymerase Inhibitors on Binding and Chain Initiation.- Zur molekularen Wirkungsweise einiger matrizeninaktivierender Antibiotika.- Discussion.- II. Protein Synthesis.- Localization of Streptomycin-Resistance in the 30 s Ribosome of E. coli. Mode of Action of Cycloheximide.- Inhibitors as Tools in Elucidating Ribosomal Function.- Discussion.- Inhibitors as Tools in Elucidating Hormone Action.- Effects of Cortisol and Actinomycin D on RNA Polymerase Activity of Rat Liver Nuclei: Importance of Sequence of Administration.- Discussion.- III. Supramolecular Structures.- Incorporation of Amino Acids into Mitochondrial Membrane Proteins.- Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Amino-Acids into Isolated Mitochondria from Neurospora crassa.- On the Effects of Antibiotics and Intercalating Dyes on Mitochondrial Biosynthesis.- Discussion.- Morphogenetic Processes in Acetabularia.- Discussion.- Penicillin-Sensitive Enzymatic Reactions in Bacterial Cell Wall Synthesis.- In Vivo Action of Penicillin on Cell Walls of Proteus Mirabilis.- Inhibition of Bacterial Peptidoglycan Synthesis by Bacitracin.- Discussion.- IV. Respiratory Chain.- Mechanisms and Inhibitors Acting on Electron Transport and Energy Conservation between NADH and the Cytochrome Chain.- Fuscin, an Inhibitor of NADH Dehydrogenase.- On the Possible Role of Cytochrome B-Type Pigments in Redox Relations between Mitochondria and Endoplasmic Reticulum.- Discussion.- Applications of Oligomycin and Related Inhibitors in Bio-energetics.- Inhibition of Mitochondrial Oxidative Phosphorylation by Aurovertin.- Discussion.- Inhibitors Around the Antimycin-Sensitive Site in the Respiratory Chain.- V. Cytoplasmic Compartmentation.- The Inhibition of Adenine Nucleotide Translocation by At-ractyloside.- Atractyligenin and Structural Analogues.- Elucidation of a Carrier Site for Adenine Nucleotide Translocation in Mitochondria with the Help of Atractyloside.- Discussion.- Transport and Exchange of Anions in Mitochondria.- Possible Action of n-Butylmalonate on Hydrogen Transport in Perfused Liver.- Discussion.- Cation Specificity of Inhibitors.- Stereochemical Aspects of Macrotetrolide Action and Biosynthesis.- Nuclear Magnetic Resonance Studies of Valinomycin, Alame-thicin, and Gramicidin S, and their Interaction with Phospholipids.- Discussion.- Site-Specific Inhibitors of Gluconeogenesis.- Discussion.- VI. Appendix.- Chemical Structures of Inhibitors.