Inflammation and Cancer, Volume 1: Methods and Protocols: Experimental Models and Practical Approaches

ISBN-13: 9781934115145 / Angielski / Twarda / 2009 / 378 str.

Serguei V. Kozlov

Opis

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. In Inflammation and Cancer: Methods and Protocols, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology, selectively focused on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis. Volume 1, Experimental Models and Practical Approaches provides an overview of a spectrum of techniques developed to analyze the outcomes of inflammation-mediated carcinogenesis on the tissue, cellular, and molecular levels while highlighting several diagnostic aspects, such as biomarker discovery and molecular signatures evaluation. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step laboratory protocols, and a notes section, which examines tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Inflammation and Cancer: Methods and Protocols promises to serve as a vital guide and resource for investigators and clinicians working toward the goal of combatting the estimated 2, 200 inflammation-related oncogenesis fatalities occurring every day."

Spis treści

Part I: Experimental Approaches to Study Chronic Inflammation-Related Carcinogenesis 1. Collection and Preparation of Rodent Tissue Samples for Histo-Pathological and Molecular Studies in Carcinogenesis Yelena Golubeva and Keith Rogers 2. Biomarkers of Cell Proliferation in Carcinomas: Detection of Angiogenesis and Infiltrated Leukocytes Coen I.M. Baeten 3. Assessment of Local Proteolytic Milieu as a Factor in Tumor Invasiveness and Metastasis Formation: In vitro Collagen Degradation and Invasion Assays Brenda L. Petrella 4. Cytokine Multiplex Analysis Howard A. Young 5. Approaches to Determine Expression of Inflammatory Cytokines Derk Amsen, Karin E. de Visser, and Terrence Town 6. Selective Immortalization of Tumor-Specific T Cells to Establish Long-Term T Cell Lines Maintaining Primary Cell Characteristics Eugene V. Barsov 7. YKL-40: A Novel Marker Shared by Chronic Inflammation and Oncogenic Transformation Anne Roslind and Julia S. Johansen 8. Angiogenesis Links Chronic Inflammation with Cancer Hanako Kobayashi and P. Charles Lin 9. Inflammatory Bowel Disease: A Model of Chronic Inflammation-Induced Cancer Guang-Yu Yang, Sofia Taboada, and Jie Liao Part II: Oncogenic Potential of Inflammation Induced by Viral and Bacterial Infections 10. Gastric Carcinogenesis and Helicobacter pylori Infection Christine Varone, Jean-François Mosnier, Philippe Lehours, Tamara Matysiak-Budnik, and Francis Mégraud 11. Helicobacter-Based Mouse Models of Digestive System Carcinogenesis Arlin B. Rogers and JeanMarie Houghton 12. Screening for Molecular Markers of Cervical Papillomavirus Infection: Overview of Methods and Their ClinicalImplications Donna Dehn, Adriann Taylor, Timothy J. Fischer, Douglas P. Malinowski, and Kenneth R. Shroyer 13. Screening for Epstein-Barr Virus in Hodgkin’s Lymphoma Gulfaraz Khan 14. A Hepatitis C Virus Xenograft Mouse Efficacy Model Qing Zhu and Amy J. Weiner 15. Gene Expression Profiling in Cervical Cancer: Identification of Novel Markers for Disease Diagnosis and Therapy Cara M. Martin, Katharine Astbury, Lynda McEvoy, Sharon O’Toole, Orla Sheils, and John J. O’Leary 16. Detection and Genotyping Analysis of Human Papillomavirus Isolates from Liquid-Based Cervical Cytology Specimens Patricia Avissar and Douglas P. Malinowski