In Vivo NMR Imaging: Methods and Protocols

ISBN-13: 9781493961726 / Angielski / Miękka / 2016 / 753 str.

Leif Schroder; Cornelius Faber

Opis

Nuclear magnetic resonance imaging is now an indispensible diagnostic tool with resolution down to the molecular level. This valuable new text includes cutting-edge methods and protocols and describes both underlying principles and applications of NMR.

Spis treści

Part I. Basics of MRI             

 

1. Physical Basics of NMR                                                                            

            Rolf Pohmann

 

2. Spatial Encoding - Basic Imaging Sequences                                            

            Rolf Pohmann

 

3. Basic Contrast Mechanisms                                                                       

            Leif Schröder and Cornelius Faber                

 

4. Scanner Components                                                                                 

            Volker Behr

 

5. Small Animal Preparation and Handling in MRI                          

            Patrick McConville

 

Part II. Special Techniques                

 

6. Cerebral Perfusion MRI in Mice                

            Frank Kober, Guillaume Duhamel, and Virginie Callot

 

7. High Field Diffusion Tensor Imaging in Small Animals and Excised Tissue

            Bernadette Erokwu, Chris Flask, and Vikas Gulani

 

8. The BOLD Effect                                                                                      

            Joan M. Greve

 

9. Screening of CEST MR Contrast Agents              

            Xiaolei Song, Kannie W.Y. Chan, and Michael T. McMahon

 

10. Hyperpolarized Noble Gases as Contrast Agents            

            Xin Zhou

 

11. Hyperpolarized Molecules in Solution                                                     

            Jan Henrik Ardenkjaer-Larsen, Haukur Jóhannesson, Stefan J. Petersson, and Jan Wolber 

 

12. MR Oximetry                   

            Jeff F. Dunn

 

13. MRI using Intermolecular Multiple-Quantum Coherences         

            Rosa T. Branca

                       

Part III. Brain Applications

 

14. Experimental Stroke Research: The Contributions of In vivo MRI   

            Therése Kallur and Mathias Hoehn

 

15. Volumetry and Other Quantitative Measurements to Assess the Rodent Brain

            Alize Scheenstra, Jouke Dijkstra, and Louise van der Weerd

 

16. Models of Neurodegenerative Disease - Alzheimer's Anatomical and Amyloid Plaque Imaging          

            Alexandra Petiet, Benoit Delatour, and Marc Dhenain

 

17. MRI in Animal Models of Psychiatric Disorders

            Dana S. Poole, Melly S. Oitzl, and Louise van der Weerd

 

18. Spectroscopic Imaging of the Mouse Brain         

            Dennis W. J. Klomp and W. Klaas Jan Renema

 

Part IV. Spine Applications

                       

19. Spinal Cord – MR of Rodent Models     

            Virginie Callot, Guillaume Duhamel, and Frank Kober

 

Part V. Cardiovascular Applications

 

20. Assessment of Cardiac Function

            Jürgen E. Schneider

 

21. Plaque Imaging in Murine Models of Cardiovascular Disease    

            Gert Klug, Volker Herold, and Karl-Heinz Hiller

 

22. Interventional MRI in the Cardiovascular System          

            Harald H. Quick

 

23. MR for the Investigation of Murine Vasculature

            Christoph Jacoby and Ulrich Flögel

 

Part VI. Lung Applications               

 

24. MRI of the Lung: Non-invasive Protocols and Applications to Small Animal Models of Lung Disease

            Magdalena Zurek and Yannick Crémillieux

 

Part VII. Cancer Models                   

 

25. Characterization of Tumor Vasculature in Mouse Brain by USPIO Contrast-enhanced MRI   

            Giulio Gambarota and William Leenders

 

26. Cancer Models - A Multiparametric Application of MRI in Rodent Hepatic Tumor Model      

            Feng Chen, Frederik De Keyzer, and Yicheng Ni

 

Part VIII. Functional MRI                

 

27. BOLD MRI Applied to a Murine Model of Peripheral Artery Disease  

            Joan M. Greve

 

28. Manganese-enhanced Magnetic Resonance Imaging

            Susann Boretius and Jens Frahm

 

29. Spin Echo BOLD fMRI on Songbirds    

            Colline Poirier and Anne-Marie Van der Linden

 

Part IX. Phenotyping             

 

30. MRI to Study Embryonic Development 

            Bianca Hogers

 

31. Mouse Phenotyping with MRI                                                                            

            X. Josette Chen and Brian J. Nieman

 

32. Analysis of Freshly Fixed and Museum Invertebrate Specimens using High-resolution, High-throughput MRI                       

            Alexander Ziegler and Susanne Mueller

 

Part X. Metabolic and Targeted Imaging                  

 

33. Applications of Hyperpolarized Agents in Solutions                              

            Jan Henrik Ardenkjaer-Larsen, Haukur Jóhannesson, J. Stefan Petersson, and Jan Wolber

 

34. Target-specific Paramagnetic and Superparamagnetic Micelles for Molecular MR Imaging                  

            Roel Straathof, Gustav J. Strijkers, and Klaas Nicolay

 

35. Tracking Transplanted Cells by MRI - Methods and Protocols              

            Michel Modo

 

36. MRI of CEST Based Reporter Gene                   

            Guanshu Liu and Assaf A. Gilad

Blurb

Nuclear magnetic resonance imaging represents a technique that is indispensable in every day biomedical diagnostics. Thanks to the numerous ways to manipulate and detect an NMR signal, it is possible to obtain a variety of information with excellent spatial and temporal resolution. Today’s MRI techniques go far beyond the illustration of pure anatomical structures and include the revealing of processes down to the molecular level. The number of small animal imaging centers relying on MRI as a key method for preclinical research to understand diseases and to test for novel treatments is growing rapidly. In Vivo NMR Imaging: Methods and Protocols is written as an experimental laboratory text to provide a descriptive approach of the various applications of magnetic resonance imaging and its underlying principles. Starting from a compact introduction of basic NMR physics and image encoding techniques suitable for a broad audience in the life sciences, the concept focuses on addressing the many ways of generating contrast in MR images. The authors cover an interdisciplinary range of problems to be addressed by this non-invasive modality, including study protocols for addressing morphological, physiological, functional, and biochemical aspects of various tissues in living organisms. Information about practical aspects of designing experimental studies that follow the special conditions for micro imaging setups are also provided.

 

Written in the successful Methods in Molecular Biology™ series format, In Vivo NMR Imaging: Methods and Protocols aims to be an experimental compendium of modern in vivo MR imaging with special focus on recent developments in molecular imaging and new protocols for imaging metabolism and molecular markers.