In Vivo Fluorescence Imaging: Methods and Protocols

ISBN-13: 9781493937196 / Angielski / Twarda / 2016 / 213 str.

Mingfeng Bai

Opis

This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking, image-guided treatment, and fluorescence imaging in the second NIR window, as well as protocols for evaluation methods before and after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous fields who wish to become more familiar with in vivo fluorescence imaging techniques.

Spis treści

1. Before In Vivo Imaging: Evaluation of Fluorescent Probes using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays

            Shaojuan Zhang

 

2. Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes

            Xiaoxi Ling

 

3. In Vivo Fluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models

            Jie Cao and Mingzhou Zhou

 

4. FRET Imaging of Enzymatic Activities Using Smart Probes

            Jinbo Li, Yan Zhang, and Zhen Cheng

 

5. Fluorescence Imaging of Inflammation in Live Animals

            Mingzhou Zhou, Jie Cao, and Walter J. Akers

 

6. Fluorescence Lifetime Imaging of Cancer In Vivo

            Owen Peng and Walter J. Akers

 

7. Non-Invasive Imaging of Fluorescent Reporters in Small Rodent Models Using Fluorescence Molecular Tomography

            Weizhou Hou and Steve H. Thorne

 

8. In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

            Rui Huang, Peter S. Conti, and Kai Chen

 

9. Intraoperative Fluorescence Imaging and Multimodal Surgical Navigation Using Goggle System

            Christopher A. Mela, Francis A. Papay, and Yang Liu

 

10. Utilization of Near-Infrared Fluorescent Imaging for Pharmaceutically Relevant Applications

            Jelena M. Janjic, Sravan Kumar Patel, and Christina Bagia

 

11. In Vivo Cell Tracking Using Two-Photon Microscopy

            Daniela Malide

 

12. Small Animal Imaging Using Diffuse Fluorescence Tomography

            Scott C. Davis and Kenneth M. Tichauer

 

13. In Vivo Metal Ion Imaging Using Fluorescent Sensors

            Genevieve C. Van de Bittner and Tasuku Hirayama

 

14. Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo

            Qiaoya Lin, Huang Huang, Juan Chen, and Gang Zheng

 

15. In Vivo Fluorescence Imaging in the Second Near-Infrared Window Using Carbon Nanotubes

            Guosong Hong and Hongjie Dai

 

16. Ex Vivo Imaging, Biodistribution, and Histological Study in Addition to In Vivo Imaging

            Shaojuan Zhang

 

17. Experimental Design and Data Analysis of In Vivo Fluorescence Imaging Studies

            Ying Ding and Hui-Min Lin