In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols

ISBN-13: 9781617797965 / Angielski / Twarda / 2012 / 269 str.

Robert M. Hoffman

Opis

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

Recenzje

From the reviews:

"Robert M. Hoffman introduces In vivo Cellular Imaging Using Fluorescent proteins, the eighteen chapters book dedicated to the description of how fluorescence proteins have changed the way to analyze cellular processes in vivo. ... This book is very well structured with several colored figures ... . this is a useful book not only for those who study the biology of different cancer cells, but also for those who are interested in following the in vivo dynamic of cellular processes." (Manuela Monti, European Journal of Histochemistry, Vol. 56, 2012)

Spis treści

1. Assessing Cancer Cell Migration And Metastatic Growth In Vivo In The Chick Embryo Using Fluorescence Intravital Imaging

                Hon Sing Leong, Ann F. Chambers, and John D. Lewis

 

2. The Use Of Fluorescent Proteins For Intravital Imaging Of Tumor Cell Invasion

                James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg,  Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall

 

3. High Resolution In Vivo Imaging Of Fluorescent Proteins Using Window Chamber Models

                Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst

 

4. In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models

                Michael Bouvet and Robert M. Hoffman

 

5. Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease

                Zeqian Yu, Jiahua Zhou, and Robert M. Hoffman

 

6. Non-Invasive And Real-Time Fluorescence Imaging of Peritoneal Metastasis In Nude Mice

                Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, Yasuhiro Kodera

 

7. Three-Dimensional  In Vivo Imaging Of Tumors Expressing  Red Fluorescent Proteins

                Alexander P. Savitsky, Irina G. Meerovich, Viktoria V. Zherdeva, Lyaisan R. Arslanbaeva, Olga S. Burova, D.V. Sokolova. E.M. Treshchalina, Anatoly Y. Baryshnikov, Ilya I. Fiks, Anna G. Orlova, Mikhail S. Kleshnin, Ilya V. Turchin, and Alexander M. Sergeev

 

8. Real Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anti-Cancer Therapies

                H. Rosie Xing and Qingbei Zhang

 

9. In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus

                Toshiyoshi Fujiwara

 

10. Real-Time Fluorescent Imaging of Abdominal, Pleural, and Lymphatic Metastases

                Susanne Carpenter and Yuman Fong

 

11. Real-Time Imaging Of Tumors Using Replication-Competent Light-Emitting Microorganisms

                Yong A. Yu, Stephanie Weibel, Aladar A. Szalay

 

12. GFP-Transgenic Animals For In Vivo Imaging: Rats, Rabbits And Pigs

                Takashi Murakami and Eiji Kobayashi

 

13. The Use Of Fluorescent Proteins For Developing Cancer-Specific Target Imaging Probes

                Thomas McCann, Nobuyuki Kosaka, Peter L. Choyke, and Hisataka Kobayashi

 

14. In Vivo Imaging Of The Developing Mouse Embryonic Vasculature

                Irina V. Larina and Mary E. Dickinson

 

15. Screening Reef Corals For Novel GFP-Type Fluorescent Proteins By Confocal Imaging

                Anya Salih

 

16. What Does It Take To Improve Existing Fluorescent Proteins For In Vivo Imaging Applications?

                Marc Zimmer

 

17. In Vivo Imaging of Oligonucleotide Delivery

                Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, and Takahiro Ochiya

 

18. Subcellular Imaging In Vivo:  The Next GFP Revolution

                Robert M. Hoffman