In Vitro Models for Stem Cell Therapy: Methods and Protocols

ISBN-13: 9781071612279 / Angielski / Miękka / 2022

Stock, Peggy

Opis

This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I Introduction

1. Consistent Inclusion of Mesenchymal Stem Cells into In Vitro Tumor Models
Luís P. Ferreira, Vítor M. Gaspar, and João F. Mano

2. Pluripotent Stem Cells for Cell Therapy
Insa S. Schroeder

Part II Disease Modeling

3. In Vitro Methods for the Study of Glioblastoma Stem-Like Cell Radiosensitivity
Joseph H. McAbee, Charlotte Degorre-Kerbaul, and Philip J. Tofilon

4. Bioimaging of Mesenchymal Stem Cells Spatial Distribution and Interactions with 3D In Vitro Tumor Spheroids
Luís P. Ferreira, Vítor M. Gaspar, and João F. Mano

5. Investigation of the MSC Paracrine Effects on Alveolar-Capillary Barrier Integrity in the In Vitro Models of ARDS 
Johnatas Dutra Silva and Anna D. Krasnodembskaya

6. In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGFβ1-induced Pulmonary Fibrosis
Ying-Wei Lan, Chuan-Mu Chen, and Kowit-Yu Chong

7. Study of Mesenchymal Stem Cell-Mediated Mitochondrial Transfer in In Vitro Models of Oxidant-Mediated Airway Epithelial and Smooth Muscle Cell Injury
Charalambos Michaeloudes, Xiang Li,  Judith C.W. Mak, and Pankaj K. Bhavsar

8. Co-Culture of Peripheral Blood Mononuclear Cells and Endothelial Colony Forming Cells from Cord Blood of Preterm Born Babies
Jan Baier, Anchang Charles Gwellem, Roland Haase, Ines Volkmer, Babette Bartling, and Martin S. Staege

9. Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells’ Paracrine Properties
Ivan Fernandez-Bueno and  Ricardo Usategui-Martin 

10. Ex Vivo Normothermic Hypoxic Rat Liver Perfusion Model: An Experimental Setting for Organ Recondition and Pharmacological Intervention 
Federica Rigo, Victor Navarro-Tableros, Nicola De Stefano, Alberto Calleri, and Renato Romagnoli

11. Co-Culture of Human Mesenchymal Stromal Cells and Primary Mouse Hepatocytes
Mei-Ju Hsu, Madlen Christ, and Bruno Christ

12. A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease
Susanna Schubert, Luisa Brandt, and Janina Burk

13. Generation of Epidermal Equivalents from Hair Follicle Melanocytes, Keratinocytes, and Dermal Fibroblasts
Vuk Savkovic, Marie Schneider, Hanluo Li, Jan-Christoph Simon, Mirjana Ziemer, and Bernd Lethaus

Part III Characterization and Pre-Conditioning

14. Using Gene Expression Music Algorithms (GEMusicA) for the Characterization of Human Stem Cells
Martin Sebastian Staege

15. Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis
María Isabel Guillén, Alvaro Compañ, and María José Alcaraz

16. Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development
Matthias Jung, Jovita Schiller, Carla Hartmann, Jenny Pfeiffer, and Dan Rujescu

17. Engineered Tissues Made from Human iPSC-derived Schwann Cells for Investigating Peripheral Nerve Regeneration In Vitro
Rebecca Powell and James B. Phillips

18. Modulation of a Stem Cell Gene: LGR4 Knockout in a Human Cell Line by CRISPR/Cas Method
Swetlana Rot and Matthias Kappler

19. In Vitro Tool: 3D Cell Culture of Human Adipose Tissue-Derived Mesenchymal Stromal Cells on Low Stiffness Silicone Scaffolds
Peggy Stock