In Vitro Differentiation of T-Cells: Methods and Protocols

ISBN-13: 9781493997305 / Angielski / Miękka / 2020 / 267 str.

Shin Kaneko

Spis treści

1. Flow Cytometry Analysis to Identify Human CD8⁺ T-Cells

            Jacqueline Flynn and Paul Gorry

 

2. Flow Cytometry Analysis to Identify Human CD4⁺ T-Cell Subsets

            Jacqueline Flynn and Paul Gorry

 

3. Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T-Cells Redirected with Antigen-Specific Receptors

            Toshiki Ochi, Masaki Maruta, and Naoto Hirano

 

4. In Vitro Conversion of Activated T-Cells into Stem Cell Memory-Like T-Cells

            Taisuke Kondo, Yuuki Imura, Makoto Ando, Shunsuke Chikuma, and Akihiko Yoshimura

 

5. Human iPSC Generation from Antigen-Specific T-Cells

            Toshinobu Nishimura, Yukiko Murmann, and Hiromitsu Nakauchi

 

6. In Vitro Differentiation of T-Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

            Shoichi Iriguchi and Shin Kaneko

 

7. Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC

            Yohei Kawai and Shin Kaneko

 

8. In Vitro Differentiation of T-Cell: From Human iPS Cells in Feeder Free Condition

            Yutaka Yasui, Yasumichi Hitoshi, and Shin Kaneko

 

9. Differentiating CD8ab T-Cells from TCR Transduced iPSCs for Cancer Immunotherapy

            Atsutaka Minagawa and Shin Kaneko

 

10. In Vitro Differentiation of T-Cell: From CAR-Modified T-iPSC

            Tatsuki Ueda and Shin Kaneko

 

11. In Vitro Differentiation of T-Cells: From Non-Human Primate Induced Pluripotent Stem Cells

            Shoichi Iriguchi and Shin Kaneko

 

12. An Improved Method to Produce Clinical Scale Natural Killer Cells from Human Pluripotent Stem Cells

            Huang Zhu and Dan S. Kaufman

 

13. In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T-Cells

            Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima, Hideki Ohdan, Shin Kaneko, and Yasushi Uemura

 

14. In Vitro Differentiation of T-Cells from Murine Pluripotent Stem Cells

            Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song

 

15. Clonogenic Culture of Mouse Thymic Epithelial Cells

            Miho Sekai, Jianwei Wang, and Yoko Hamazaki

 

16. Single-Cell Transcriptome Analysis of T-Cells

            Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, and Fabio Luciani

 

17. Structural Modeling of Lymphocyte Receptors and Their Antigens

            Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila, Zichang Xu, Kazutaka Katoh, and Daron M. Standley

 

18. Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers

            Tinhinane Fali, Camille K'Ros, Victor Appay, and Delphine Sauce

 

19. Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

            Hideyuki Oguro

 

20. Using the Inducible Caspase9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking

            Jonathan Villanueva, Toshinobu Nishimura, and Hiromitsu Nakauchi

Blurb

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.