In Vitro Culture of Higher Plants

ISBN-13: 9780792345275 / Angielski / Miękka / 1997 / 348 str.

R. L. Pierik; R. L. M. Pierik

Opis

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects.
Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Spis treści

1. Preface. 2. Introduction. 3. History. 4. Types of Cultures. 5. Laboratory Equipment. 6. Preparation and Composition of Nutrient Media. 7. Closure of Test Tubes and Flasks. 8. Care of Plant Material. 9. Sterilization of Plant Material. 10. Isolation, Inoculation and Subculturing. 11. Mechanization. 12. The Influence of Plant Material on Growth and Development. 13. The Influence of Physcial Factors on Growth and Development. 14. The Transfer from Nutrient Medium to Soil. 15. Aids to Study. 16. Embryo Culture. 17. Germination of Orchid Seeds. 18. Vegetative Propagation of Orchids. 19. Production of Disease-Free Plants. 20. Vegetative Propagation. 21. Somaclonal Variation. 22. Test Tube Fertilization. 23. Production of Haploids. 24. Genetic Manipulation. 25. Miscellaneous Applications. 26. In vitro Cloning of Plants in the Netherlands. 27. Index. 28. Literature Cited.