In Situ Molecular Pathology and Co-Expression Analyses

ISBN-13: 9780124159440 / Angielski / Twarda / 2013 / 289 str.

Gerard Nuovo

Opis

In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework.

• Includes over 200 easy-to-follow experimental protocols
• Features chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the field
• Offers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses

Recenzje

"If you perform this type of testing in your laboratory, you must get this book. When you think all experimental approaches for in situ molecular testing methods have failed, have faith and refer to this book for the solution." --Doody.com, October 25, 2013

"Nuovo presents a textbook on in-situ-based molecular pathology for readers with minimum prior knowledge of molecular pathology in general and in-situ-based molecular pathology in particular." --Reference and Research Book News, August 2013

Spis treści

1. Introduction

2. The Basics of Molecular Pathology

3. The Biochemical Basis of In Situ Hybridization and Immunohistochemistry

4. The Basics of In Situ Hybridization

5. The Basics of Immunohistochemistry

6. The Basics of Histologic Interpretations of Tissues

7. The Recommended Protocol for In Situ Hybridization

8. The Recommended Protocol for Immunohistochemistry

9. Co-expression Analyses

10. PCR In Situ Hybridization and RT In Situ PCR