In Situ Hybridization in Brain

ISBN-13: 9781461594888 / Angielski / Miękka / 2012 / 300 str.

George Uhl

Opis

Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain.

Spis treści

Section I: In Situ Hybridization: Approaches and Applications.- 1. Cellular Location and Regulation of Proenkephalin mRNA in Rat Brain.- 2. Vasopressin and Somatostatin mRNA In Situ Hybridization.- 3. Opioid Peptides and Vasopressin: The Application of In Situ Hybridization to Studies of the Hypothalamus and Pituitary.- 4. Detection of Neuropeptide mRNAs by In SituHybridization Histochemistry.- 5. In Situ Hybridization as a Quantitative Autoradiographic Method: Vasopressin and Oxytocin Gene Transcription in the Brattleboro Rat.- 6. Glucocorticoid Regulation of Vasopressin Messenger RNA.- 7. Neuro Anatomical and Developmental Expression of Novel Brain mRNAs Determined by In Situ Hybridization.- 8. In Situ Detection of GAD mRNA in Mouse Brain.- 9. The Cellular Localization of Preprotachykinin, Preproenkephalin A and Beta Prepronerve Growth Factor mRNA.- Discussion: Nerve Growth Factor Gene Expression in Mouse Hippocampal Formation.- 10. Molecular Approaches to Human Neurological Diseases and Their Animal Models.- 11. In Situ Hybridization of Somatostatin and Vasoactive Intestinal Peptide mRNA in the Rat Nervous System: Contrasting Patterns of Ontogeny.- 12. Use of In Situ Hybridization Histochemistry to Analyze Gene Transcription in Individual Cells.- 13. In Situ Hybridization for Detection of Viral Nucleic Acid in Cell Cultures and Tissues.- Discussion: Detection of Viral Nucleic Acids in the Central Nervous System.- Section II: Methodological Issues.- 14. Probes.- (A) General Features.- (B) Nick-Translation.- (C) Single-Stranded End-Labeled DNA Probes.- (1)W. Scott Young, III.- (2)Leonard G. Davis, Michael E. Lewis and Frank Baldino, Jr..- (D) Single-Stranded Multiply-Labeled cDNA Probes.- (1) Syn Probes.- (2) Single-Stranded DNA Probes Using an M13 Template.- (E) Single-Stranded Multiply-Labeled RNA Probes.- (F) Biotin Labeling.- 15. Hybridization and Wash Conditions.- 16. Quantitative In Situ Hybridization and Determination of mRNA Content.- (1)W. Scott Young, III.- (2)Michael J. Kuhar.- 17. In Situ Hybridization Histochemistry as a Supplement to Immunohistochemistry.- 18. Determination of Specificity in In Situ Hybridization.- Section III: Appendix.- Index and Glossary.