In Situ Hybridization Protocols

ISBN-13: 9781493914586 / Angielski / Twarda / 2014 / 275 str.

Boye Nielsen

Opis

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

Spis treści

1. Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae

Ricardo Fuentes and Juan Fernández

 

2. MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos

Karen Goossens, Luc Peelman, and Ann Van Soom

 

3. Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies

Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, and Luc Leyns

 

4. Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian

Agnieszka Rybak-Wolf and Jordi Solana

 

5. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae

Bernard Thisse and Christine Thisse

 

6. LNA-based in Situ Hybridization Detection of mRNAs in Embryos

Diana K. Darnell and Parker B. Antin

 

7. Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA™ Probe Technology

Boye Schnack Nielsen, Trine Møller, and Kim Holmstrøm

 

8. MicroRNA In Situ Hybridisation in Tissue Microarrays

Julia J. Turnock-Jones and John P.C. Le Quesne

 

9. Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections

Asli Silahtaroglu

 10. Sensitive and Specific in situ Hybridisation for Early Drug Discovery

Pernille A. Usher, Elisabeth D. Galsgaard, Kimberly Kruse, Jishu Wang, Berit O. Krogh, Jette Mandelbaum, and Kasper Almholt

 

11. Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections

Electra Stylianopoulou, George Skavdis and Maria Grigoriou

 

12. Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope

Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma

 

13. Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens

Lorenzo F. Sempere

14. Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues

Neil Renwick, Pavol Cekan, Claudia Bognanni, and Thomas Tuschl

 

15. Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris^® RNA Fluorescence in situ Hybridization in Adherent Cells

Sally R. Coassin, Arturo V. Orjalo, Jr., Sheila J. Semaan, and Hans E. Johansson

 

16. Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope

Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Allissa Nielson, Hong-Thuy Vo, Yuling Luo, and Xiao-Jun Ma

 

17. Identification of Low Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia

Sergei Zolotukhin

 

18. In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes

Kathleen T. Montone

 

19. In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based Tissue Extraction from Thin Slide-mounted Sections for DNA Methylation Analysis

Lawrence S. Own and Paresh D. Patel

 

20. miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH

Meiye Wu, Matthew E. Piccini, and Anup K. Singh

 

21. PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples

Mustafa Fazli, Thomas Bjarnsholt, Niels Høiby, Michael Givskov, and Tim Tolker-Nielsen