Immunotoxicity Testing: Methods and Protocols

ISBN-13: 9781493993222 / Angielski / Miękka / 2019 / 412 str.

Jamie C. DeWitt; Cheryl E. Rockwell; Christal C. Bowman

Spis treści

Part I: Overview and Health Risk Considerations

 

1. Immunotoxicology: A Brief History

            Kenneth L. Hastings

 

2. Clinical Immunotoxicology

            L. Peyton Myers

 

3. Investigative Immunotoxicology

            Stacey E. Anderson and Hillary L. Shane

 

4. Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing

            Annalise N. vonderEmbse and Jamie C. DeWitt

 

5. Markers of Inflammation

            Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, and Ellen Evans

 

Part II: Legacy Protocols: In Vivo

 

6. The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)

            Gregory S. Ladics

 

7. Methylated-Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice

            Jessica Margaret Sido

 

8. Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment

            David M. Lehmann

 

9. Host Resistance Assays

            Stefanie C.M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, and Robert W. Luebke

 

10. Enhanced Histopathology Evaluation of Lymphoid Organs

            Susan A. Elmore

 

11. Tumor Challenges in Immunotoxicity Testing

            Sheung Ng and Judith T. Zelikoff

 

Part III: Legacy Protocols: Ex Vivo and In Vitro

 

12. Flow Cytometry for the Immunotoxicologist

            Scott T. Espenchied, Robert M. Tighe, and Kymberly M. Gowdy

 

13. Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T Lymphocyte Assay

            Gary R. Burleson, Florence G. Burleson, and Rodney R. Dietert

 

14. Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing

            Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, and Prakash S. Nagarkatti

 

15. Natural Killer (NK) Cell Assays in Immunotoxicity Testing

            Qing Li

 

16. Dendritic Cell Assays

            Joanna M. Kreitinger and David M. Shepherd

 

17. Evaluating Macrophages in Immunotoxicity Testing

            Jennifer Franko, Jamie L. McCall, and John B. Barnett

 

18. Evaluating Cytokines in Immunotoxicity Testing

            Emanuela Corsini and Robert V. House

 

Part IV: Alternative Protocols

 

19. Functional Assays of Hematopoietic Stem Cells in Toxicology Research

            Michael D. Laiosa

 

20. CD4⁺ T Cell Differentiation and Activation

            Jim Reed and Scott A. Wetzel

 

21. Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing

            Elia D. Tait Wojno and Celine A. Beamer

 

22. Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay

            Marsha D.W. Ward and Lisa B. Copeland

 

Part V: Emerging Issues

 

23. Challenges for Integrating Immunotoxicology into the 21^st Century Toxicology Testing Paradigm

            Peer W.F. Karmaus and Agnes L. Karmaus

Blurb

This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed.  Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.