Immunophenotyping: Methods and Protocols

ISBN-13: 9781493996490 / Angielski / Twarda / 2019 / 356 str.

J. Philip McCoy Jr

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A Practical Guidebook

Florian Mair and Aaron J. Tyznik

2. Immunophenotyping by Mass Cytometry

Gregory K. Behbehani

3. Fluorescent Cell Barcoding for Immunophenotyping

Valentina Giudice. Giovanna Fantoni, and Angélique Biancotto

4. Immunophenotyping using Dried and Lyophilized Reagents

Marc Langweiler

5. Guidelines for Gating Flow Cytometry Data for Immunological Assays

Janet Staats, Anagha Divekar, J. Philip McCoy, and Holden T. Maecker

6. Strategies and Techniques for NK Cell Phenotyping

Chen Ziqing, Andreas Lundqvist, and Kristina Witt

7. Immunophenotyping of Human B Lymphocytes in the Blood and in the Adipose Tissue

Alain Diaz, Maria Romero, Daniela Frasca, and Bonnie B. Blomberg

8. Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells

Ankit Saxena, Pradeep K. Dagur, and Angélique Biancotto

9. Immunophenotyping of Human Regulatory T Cells

Janet Staats

10. Immunophenotyping of Human Innate Lymphoid Cells

Sara Trabanelli, Alejandra Gomez-Cadena, and Camilla Jandus

11. Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis

Abdullah Alsuwaidan, Franklin Fuda, Weina Chen, and Mingyi Chen

12. Immunophenotyping of Circulating Endothelial Cell and Endothelial Microparticles

Nicholas Wanner and Kewal Asosingh

13. Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells

Moen Sen, Ling Wang, Liping Yu, and Erica L. Carpenter

14. Quality Control of Immunophenotyping

Bruce Greig

15. Immunophenotyping of Acute Myeloid Leukemia

Pallavi Kanwar Galera, Chunjie Jiang, and Raul Braylan

16. Immunophenotyping of Acute Lymphoblastic Leukemia

Joseph A. DiGiuseppe and Jolene L. Cadinali

17. Clinical Flow Cytometry Testing in Chronic Lymphocytic Leukemia

Dalia A. Salem and Maryalice Stetler-Stevenson

18. Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)

Andrea J. Illingworth, Iuri Marinov, and D. Robert Sutherland

Blurb

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.