Immunometabolism: Methods and Protocols

ISBN-13: 9781071608012 / Angielski / Twarda / 2020 / 284 str.

Suresh Mishra

Spis treści

1. Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq

            Janilyn Arsenio

 

2. Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe

            Felipe Baeza-Lehnert, Carlos A. Flores, Anita Guequen, and L. Felipe Barros

 

3. The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level

            Iivari Kleino, Eliisa Kekäläinen, and Tapio Lönnberg

 

4. Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling

            Kevin J. Williams and Steven J. Bensinger

 

5. Proteomic Network Analysis of Polarized Macrophages

            Jayanta K. Chakrabarty, Abu Hena Mostafa Kamal, A.D.A. Shahinuzzaman, and Saiful M. Chowdhury

 

6. The Purification and Characterization of Exosomes from Macrophages

            Eran Fridman, Lana Ginini, Ziv Gil, and Neta Milman

 

7. The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry

            Inbar Azoulay-Alfaguter and Adam Mor

 

8. Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model

            Tanya N. Augustine

 

9. Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System

            Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, and Lindsay E. Robinson

 

10. A Gene Expression Analysis of M1 and M2 Polarized Macrophages

            Nour Eissa, Hayam Hussein, and Jean-Eric Ghia

 

11. Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages

            Casey M. Daniels, Arthur Nuccio, Pauline R. Kaplan, and Aleksandra Nita-Lazar

 

12. The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer

            Bridgette M. Cumming, Vineel P. Reddy, and Adrie J.C. Steyn

 

13. Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification

            Hsi-Ju Wei, John J. Letterio, and Tej K. Pareek

 

14. The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP⁺) Electrode in Isolated Mitochondria and Intact Cells

            João S. Teodoro, Ivo F. Machado, Ana C. Castela, Anabela P. Rolo and Carlos M. Palmeira

 

15. The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs)

            Jesse J.R. Masson, Matias Ostrowski, Gabriel Duette, Man kit Sam Lee, Andrew J. Murphy, Suzanne M. Crowe, and Clovis S. Palmer

 

16. The Measurement of Whole Body Glucose Homeostasis in Mice

            Yang Xin Zi Xu, Sudharsana R. Ande, and Suresh Mishra

 

17. Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy

            Andrew D. Sheppard and Joanne Lysaght

 

18. Sex Differences in Immunometabolism: An Unexplored Area

            Suresh Mishra, Geetika Bassi, and Yang Xin Zi Xu

 

19. Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis

            Nnamdi Ikeogu, Chidalu A. Edechi, Gloria Akaluka, Aida Feiz-Barazandeh, and Jude E. Uzonna

Blurb

This detailed book showcases the tremendous effort and progress made in developing techniques and protocols for the study of immunometabolism, and in utilizing recent technological advances for probing and manipulating adipose and immune cells, and subsequently, their functions and immunometabolic consequences. Written by experts in the field, many chapters use macrophages as a model immune cell type, due to their prominence in the innate immune system and the exhaustive study of their traits. Protocols using adipocytes, dendritic cells, and T cells as model cell lines, as well as measurement of glucose metabolism at the systemic level, have also been included. Written for the highly successful Methods in Molecular Biology book series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunometabolism: Methods and Protocols serves as a vital guide for researchers working at the important interface of immunology and metabolism.