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Immunology: Overview and Laboratory Manual
ISBN-13: 9783030646851 / Angielski / Twarda / 2021 / 471 str.
Kategorie BISAC:
Wydawca:
Springer
Język:
Angielski
ISBN-13:
9783030646851
Rok wydania:
2021
Wydanie:
2021
Ilość stron:
471
Waga:
0.85 kg
Wymiary:
23.39 x 15.6 x 2.69
Oprawa:
Twarda
Wolumenów:
01
Dodatkowe informacje:
Wydanie ilustrowane
27. Immunology laboratory policies
28. Instructions for notebook entries
29. Instructions for writing lab reports
30. Lab report topics
EXERCISE 1: Using a compound microscope
EXERCISE 2: Pipetting and dilution technique, identification of mouse lymphoid organs
EXERCISE 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing
EXERCISE 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens
EXERCISE 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells
EXERCISE 6: Clearance of bacteria from mouse blood by the RES
EXERCISE 7: Hemolytic Plaque assay (The Jeme Plaque Assay)
EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays
EXERCISE 9: Determination of Antibody Titer: Screening Techniques
EXERCISE 10: Immunodiffusion and Immunoelectrophoresis
EXERCISE 11: Protein Affinity Purification of Rabbit IgG
EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
EXERCISE 13: Immunoblotting (Western Blotting)
EXERCISE 14: Protein Assay
EXERCISE 15: Enzyme-linked Immunosorbent Assay (ELISA)
EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT)
EXERCISE 17: Immunofluorescence assay (IFA)
EXERCISE 18: Flow Cytometry
EXERCISE 19: Complement Fixation Assays
Tissue Cell Culture Techniques
EXERCISE 20: Mitogen Induced Response of Lymphocytes:
Detection of Band T cell Lymphocytes Using Immunofluorescence
EXERCISE 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells
EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production
EXERCISE 23: Macrophage Migration Inhibition
EXERCISE 24: Monoclonal Antibody Preparation
EXERCISE 25: Immunization of mice with a recombinant protein
EXERCISE 26: Polarization of T helper cells into TH1 subset
EXERCISE 27: Immunoprecipitation
EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers
EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies
Appendix
Sample Practice Questions
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