Immunologic Methods in Steroid Determination

ISBN-13: 9781468460537 / Angielski / Miękka / 2012 / 232 str.

Fernand G. Peron; Burton V. Caldwell

Opis

The two main goals of the symposium upon which this volume is based were 1) to cement together knowledge presently available in the field of antibodies to steroids and obtainable only under separate covers in different journals and books, and 2) to present new data which could lead to a more complete understanding of physiologic phenomena like those occurring during the menstrual cycle, or to the elucidation of the mechanisms involved in steroid-protection interaction, or to the practical application of immunologic techniques to measurements of steroid hor mones. These techniques are extremely sensitive and can measure levels of steroid on the same order of magnitude as the radioisotope methods. However, the latter are much more laborious and costly which limits their use in many cases to the research laboratory. But the immunologic techniques generally classified as radioimmunoassay, are fraught with difficulties and problems which must be overcome. Fortunately, perhaps, the subject of immunologic techniques as applied to steroid determination is the child of radioimmunoassay of proteins, so to speak. Many of the problems which confront the former have been resolved in the latter instance. Thus, we are in an advantageous position because we are aware of the biologic and technical problems of the earlier radioimmunoassay techniques. Similar experiences have been reported in the book about the use of immunologic techniques for determination of steroid hormones."

Spis treści

I Chemical and Immunologic Aspects of Antibodies to Steroids.- 1. Structural Basis of Antibody Specificity.- 2. Early Approaches to Production, Analysis, and Use of Steroid-Specific Antisera.- 3. Specificities of Steroid Antibodies.- II The Use of Antibodies for Radioimmunoassay of Steroid Hormones.- 4. Preparation and Purification of Antibodies to Steroids.- 5. Solid-Phase Radioimmunoassay of Serum Estradiol-17?: A Semi-Automated Approach.- 6. Radioimmunoassay of Plasma Estrogens: Use of Polymerized Antibodies.- 7. Solid-Phase Radioimmunoassay of Steroids.- 8. Hapten-Radioimmunoassay for Steroid Hormones.- 9. The Use of Affinity Chromatography in the Purification of Steroid-Binding Proteins.- III The Use of Antibodies to Steroids in Physiologic Studies.- 10. Physiologic Studies Using Antibodies to Steroids.- 11. The Use of Antibodies as a Tool in Studies of Reproductive Physiology.- Summary.- Summary.