Immunogenetics: Methods and Applications in Clinical Practice

ISBN-13: 9781617798412 / Angielski / Twarda / 2012 / 689 str.

Frank T. Christiansen; Brian D. Tait

Opis

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibility barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.

Recenzje

From the reviews:

"This book is a comprehensive volume covering a wide-range of topics related to HLA in the clinical setting. ... this book is an extremely useful reference for those working in the field of transplantation, or with an interest in the clinical applications of HLA. The introductory chapters to various topics are clear and provide a good amount of detail and are well referenced for those who wish to read further on the subject." (Vivien Hanson, Immunology News, February, 2013)

Spis treści

1. The Major Histocompatibility Complex - A Paradigm for Studies of the Human Genome

            Richard J. N. Allcock

 

2. HLA Typing by SSO and SSP Methods

            Heather Dunckley

 

3. Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity

            Michael D. Varney, Alison S. L. Castley, Katri Haimila and Päivi Saavalainen

 

4. HLA Typing Using Bead Based Methods

            Daniel Trajanoski and Samantha J. Fidler

 

5. HLA Typing by Direct DNA Sequencing

            Linda K. Smith

 

6. Data Analysis of HLA Sequencing using Assign-SBT v3.6+ from Conexio

            Carla Wirtz and David Sayer

 

7. Simple Methods for the Detection of HLA-G Variants in Coding and Non-Coding Regions

            Holger Nückel, Erick C. Castelli, Philippe Moreau, Crista Ochsenfarth, Peter A. Horn, and Vera Rebmann

 

8. Molecular Typing of HLA-E

            Nina Lauterbach, Christina E.M. Voorter, and Marcel G.J. Tilanus

 

9. Molecular Analysis of Complement Component C4 Gene Copy Number

            Alison Castley and Olga Patricia Martinez

 

10. Genotyping of Single Nucleotide Polymorphisms (SNPs) by 5’ Nuclease Allelic Discrimination

            Mari Malkki and Effie W. Petersdorf

 

11. High Resolution MICA Genotyping by Sequence-Based Typing (SBT)

            Yizhou Zou and Peter Stastny

 

12. Standard Methods for the Management of Immunogenetic Data

            Pierre-Antoine Gourraud, Jill A. Hollenbach, Thomas Barnetche, Richard M. Single, and Steven J. Mack

 

13. Analytical Methods for Immunogenetic Population Data

            Steven J. Mack, Pierre-Antoine Gourraud, Richard M. Single, Glenys Thomson, and Jill A. Hollenbach

 

14. Analytical Methods for Disease Association Studies with Immunogenetic Data

Jill A. Hollenbach, Steven J. Mack, Glenys Thomson, and Pierre-Antoine Gourraud

 

15. Impact of HLA Matching and HLA Antibodies in Organ Transplantation: a Collaborative Transplant Study View

            Caner Süsal and Gerhard Opelz

 

16. Screening for Antibodies Against MICA by Luminex Flow Cytometry

            Yizhou Zou and Peter Stastny

 

17. HLA Antibody Detection and Characterization by Solid Phase Immunoassays - Methods and Pitfalls

            Andrea A. Zachary, Renato M. Vega, Donna P. Lucas, and Mary S. Leffell

 

18. Detection and Characterisation of Alloreactive T Cells

            Mandvi Bharadwaj, Nicole A. Mifsud, and James McCluskey

 

19. Detection of Allo-HLA Crossreactivity by Viral Specific Memory T-Cell Clones using Single HLA Transfected K562 Cells

            Lloyd J. D’Orsogna, Ellen M.W. van der Meer-Prins, Yvonne M. Zoet, Dave L. Roelen, Ilias I.N. Doxiadis, and Frans H.J. Claas

 

20. Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node

            Gabriella Tassone and Samantha J. Fidler

 

21. Crossmatching by Complement-Dependent Lymphocytotoxicity (CDC)

            Samantha J. Fidler

 

22. The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation

            Jonathan Downing

 

23. Overview of the Killer Cell Immunoglobulin-Like Receptor (KIR) System

            Raja Rajalingam

 

24. KIR Typing by Non Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers (PCR-SSP)

            David Ordóñez, Manuela Moraru, Natalia Gómez-Lozano, Elisa Cisneros, and Carlos Vilches

 

25. Killer Cell Immunoglobulin-Like Receptors (KIR) Typing by DNA Sequencing

            Lihua Hou, Minghua Chen, Noriko Steiner, Kanthi Kariyawasam, Jennifer Ng, and Carolyn K. Hurley

 

26. An Overview of Methods Required to Evaluate Donor NK Cell Alloreactivity for Haploidentical Haemopoietic Stem Cell Transplantation

            Andrea Velardi

 

27. The Detection of NK Cell Alloreactivity by Flow Cytometric CD107a Assay

            Dianne De Santis, Bree Foley, Campbell S. Wit, and Frank T. Christiansen

 

28. Clinical Production and Therapeutic Applications of Alloreactive Natural Killer Cells

            David H. McKenna, Diane M. Kadidlo, Sarah Cooley, and Jeffrey S. Miller

 

29. Minor Histocompatibility Antigen Typing by DNA Sequencing for Clinical Practice in Hematopoietic Stem-Cell Transplantation

            Eric Spierings and Els Goulmy

 

30. Donor Registries and Search Strategies

            Carolyn K. Hurley, Machteld Oudshoorn, and Michelle Setterholm

 

31. Cytokine Gene Polymorphisms: Methods of Detection and Biological Significance

            Gurvinder Kaur and Narinder Mehra

 

32. IMGT® Tools for the Nucleotide Analysis of Immunoglobulin (IG) and T Cell Receptor (TR) V-(D)-J Repertoires, Polymorphisms, and IG Mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS

            Eltaf Alamyar, Patrice Duroux, Marie-Paule Lefranc, and Véronique Giudicelli

 

33. IMGT/DomainGapAlign: the IMGT® Tool for the Analysis of IG, TR, MH, IgSF and MhSF Domain Amino Acid Polymorphism

            François Ehrenman and Marie-Paule Lefranc

 

34. Human Gm, Km and Am Allotypes and their Molecular Characterization: a Remarkable Demonstration of Polymorphism

            Marie-Paule Lefranc and Gérard Lefranc

 

Blurb

The HLA molecules are important regulators of the immune response through mediating antigen presentation and interaction between key immune mediating cells. They are also the major histocompatibilty barriers to transplantation, which is the clinical paradigm of the self versus non self concept. It is now recognized that this diverse range of gene systems involved in the control of the immune response have been shown to be important in many aspects of clinical practice. As a result many new molecular and cellular methods have been developed for identifying these genes and their polymorphisms, and immunogenetic laboratories specializing in these methods have developed to support transplantation and other clinical programs. Immunogenetics: Methods and Applications in Clinical Practice focuses on methods for human clinical practice. The emphasis rests on those assays which are of established or potential clinical utility and are likely to be included in the repertoire of tests provided by a routine diagnostic and service laboratory. This volume also contains several review chapters of the MHC complex, the KIR complex, the human immunoglobulin allotypes, as well as reviews of the methods for the detection of alloreactive NK cells and the detection of HLA antibodies by solid phase assays.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Immunogenetics: Methods and Applications in Clinical Practice seeks to serve both the immunogenetics community  and the wider scientific community with a collection of detailed information and helpful tips attained by many years of experience in the field.