Immunofluorescence in Clinical Immunology: A Primer and Atlas

ISBN-13: 9783764361822 / Angielski / Twarda / 2000 / 231 str.

Wulf B. Storch; W. B. Storch; R. a. Klein

Opis

This primer on immunofluorescence techniques, which first appeared in 1979, is a richly illustrated handbook suitable for everyday practical work in the laboratory, useable as both an introduction to the subjec t as well as an atlas. In hardly any other area of medicine are there so many new findings to report. The second edition of this book is con cerned not only with the detection methods which now form an essential and established part of diagnostic techniques, but also with the most recent research results such as the discovery of antibodies against A uerbachFs plexus and against podocytes. The new edition contains many new high-quality color photomicrographs, as well as detailed descripti ons of new antibodies, for example, against AuerbachFs plexus, podocyt es, and the so-called ANCAs. Considerable attention is paid to diagnos tic methods for use with auto-immune diseases. For the first time the material has been presented in the form of a broad overview.

Spis treści

1 Introduction to the basic principles and techniques of immunofluor-escence.- 2 Production of antibodies and labelling with fluorochromes.- 3 Characterisation and quality control of antisera and conjugates.- 4 Actual optical immunofluorescence techniques.- 5 Instrumental technique and the documentation of findings.- 6 General advice.- 7 Applications of immunofluorescence.