Immunodiagnostics and Patient Safety

ISBN-13: 9783110249477 / Angielski / Twarda / 2011 / 172 str.

Krystyna Sztefko

Opis

Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Proper validation of methods and quality assurance have little effect on frequency of occurrence of false positive or false negative results, which, if unrecognized, may lead to patient's misdiagnosis, unnecessary treatment or even unnecessary surgery. Deep knowledge of basic principles of immunochemical methods (antigen-antibody reaction, standardization, matrix effect, limit of detection, cross-reactivity, etc.), sources of analyte-independent interferences (preanalitycal errors, the presence of binding proteins, the presence of autoantibodies) and analyte-dependent interferences (presence of heterophilic antibodies, high-dose effect) are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results and, at the same time, improve patient's care and patient's safety.

Today most of immunochemistry methods for the determination of proteins, peptides, drugs, and many small molecules are fully automated, with good precision, excellent sensitivity and short reaction time. However, inaccuracy due to poor standardization and the presence of interfering substances in biological samples is still a serious and life-threatening issue. Deep knowledge of basic principles of immunochemical methods, sources of analyte-independent interferences and analyte-dependent interferences are very important to understand, detect, reduce and/or eliminate the interferences. This book helps to reduce false results, improve patient's care and patient's safety.

Spis treści

Immunodiagnostics and patient safety

Table of contents
1. Overview of immunochemistry (basic principles, standardization, limit of detection, cross-reactivity, ect)
2. Interferences in immunochemistry
a. Matrix effects
b. Preanalitycal factors
c. Binding proteins
d. Macroforms of proteins
e. High-dose effect
f. Heterophilic antibody
g. Anti-animal antibody
h. Autoantibody
3. Methods of detection and reduction of interferences
4. The impact of interferences on patients safety - examples

O autorze

Krystyna Sztefko, Jagiellonian University, Krakow, Poland.