Immunocytochemistry and Related Techniques

ISBN-13: 9781493923120 / Angielski / Twarda / 2015 / 473 str.

Adalberto Merighi; Laura Lossi

Opis

This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research.

Spis treści

1. The Evolution of Immunocytochemistry in the Dissection of Neural Complexity

Adalberto Merighi and Laura Lossi

 

Part I

2. Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System

Mareike Selcho and Christian Wegener

 

3. Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris

Giovanna Ponte and Graziano Fiorito

 

4. Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain

Nobuhiko Miyasaka, Noriko Wakisaka, and Yoshihiro Yoshihara

 

Part II

5. Immunocytochemical Phenotype of Differentiating Neurons

Andrea Diana and Antonio Carai

 

6. Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus

Oliver von Bohlen und Halbach

 

7. Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogues. Benefits and Limitations

Alvaro Duque and Pasko Rakic

 8. Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous SystemArmin Schneider

 

 

Part III

9. In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and Autophagy

Maria Grazia Bottone, Francesco Paolo Fanizzi, and Graziella Bernocchi

 

10. Immunocytochemical Detection of Intraneuronal Aβ Peptides in Mouse Models of Alzheimer's Disease

Oliver Wirths and Anika Saul

 

11. Purification and Characterization of Mouse Olfactory Ensheathing Cells and their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS)

Chrystian Junqueira Alves, Alexandre Baldasserini Guimarães, Jessica Ruivo Maximino, Sergio Paulo Bydlowski, and Gerson Chadi

 

12. Immunocytochemistry of Microglial Cells

Dmitrii E. Korzhevskii, Olga Kirik, and Elena Sukhorukova

 

13. Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage

Jean-Pierre Louboutin

 

 

Part IV

14. Quantification of Immunocytochemical Colocalization in Neurons

Brad R. Rocco and Kenneth N. Fish

 

15. Immunogold Electron Microscopic Quantification of Small Molecular Compounds and Proteins at Synapses and Other Neural Profiles

Max Larsson, Linda Hildegard Bergersen, and Vidar Gundersen

 

16. Combined Immunocytochemistry and Tracing of Neural Connections

Taisuke Miyazaki and Masahiko Watanabe

 

17. Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and Electrophysiology

Alberto Granato and Andrea De Giorgio

 

18. Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices

Silvia Alasia, Adalberto Merighi, and Laura Lossi

 

19. Combined Immunochemistry and Live-Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain

Ruth M. Empson, Malinda L. S. Tantirigama, Manfred J. Oswald, Stephanie M. Hughes, and Thomas Knöpfel

 

 

Part V

20. Array Tomography: A Novel High-Resolution Immunofluorescence Technique

Mariano Soiza-Reilly

 21. Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization

Bas M. C. Cloin, Casper C. Hoogenraad, Marina Mikhaylova, and Lukas C. Kapitein

 

22. Studying Membrane Dynamics of Proteins Using Quantum Dots

Martin Heubl, and Sabine Lévi

 

23. Optical Protein Patterning

Santiago Costantino