Immune Receptors: Methods and Protocols

ISBN-13: 9781071619469 / Angielski / Miękka / 2022 / 268 str.

Jonathan Rast; Katherine Buckley

Opis

This volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The chapters in this book cover methods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Immune Receptors: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers interested in learning more about this developing field.  

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors

Jordan D. Mohr, Meraj Ramezani, David Holowka, and Barbara A. Baird

2. Low-Cost and Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance

      Kimiko Kuroki, Hideo Fukuhara, Takashi Tadokoro, and Katsumi Maenaka

3. Analysis of the Sub-Cellular Localization of NLRs Upon Shigella flexneri Infection Exemplified by NOD1

Christine Arnold, Kornelia Ellwanger, and Thomas A. Kufer

4. Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones 

Helen Dooley

5. Identification of Glycan-Specific Variable Lymphocyte Receptors using Yeast Surface Display and Glycan Microarrays

   Tanya R. McKitrick, Melinda S. Hanes, Charles S. Rosenberg, Jamie Heimburg-Molinaro, Max D. Cooper, Brantley R. Herrin, and Richard D. Cummings

6. Cell Aggregation Assays for Homophilic Interactions between Cell Surface Protein

Aidan L. Huene and Matthew L. Nicotra

7. Measuring Mutator Enzyme Activity using an E. coli-Based Colony Formation Assay

Mei-Chen Liu and Sebastian D. Fugmann

8. Lamprey Variable Lymphocyte Receptor Monoclonal Antibodies for Whole Cell Surface Antigens

Gayathri Bommakanti

9. A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assays

      Assunta Liberti, Brittany A. Leigh, Zachary Graham, and Larry J. Dishaw

10. Assessment of Scavenger Receptor Cysteine Rich Domain Binding to Bacteria

      Janice C. Telfer, Haoting Hsu, Miles Dae Tyner, and Lauren Le Page

11. Bioinformatics Approaches for Analyzing Multigene Families Encoding Immune Receptors

Katherine M. Buckley

12. Production and Characterization of SIV-Specific CAR/CXCR5 T Cells

Mary S. Pampusch, Agnes Hajduczki, Gwantwa Mwakalundwa, Elizabeth Connick, Edward A. Berger, and Pamela J. Skinner 

13. Engineering IgG1 Fc Domains that Activate the Complement System

      Chang-Han Lee and George Delidakis

14. An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phagocytosis

      Myron A. Zwozdesky, Chenjie Fei, and James L. Stafford

15. Single Cell Sequencing for Everybody

Valentina Proserpio, Vanessa Falvo, Carlotta Duval, Giacomo Donati, and Salvatore Oliviero

16. Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput Sequencing

Jennifer R. Hom, Deepak Tomar, and Christopher M. Tipton

17. Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies

Johanna S. Selvaratnam, Tracy S.H. In, and Michele K. Anderson

Subject Index List…