Immune Mediators in Cancer: Methods and Protocols

ISBN-13: 9781071602461 / Angielski / Twarda / 2020 / 373 str.

Ivana Vancurova; Yan Zhu

Spis treści

Part I: Detection of Immune Mediators in Cancer Cells and Tissues

 

1. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique

            Maria Faresjö

 

2. Analysis of Circulating HMGB1 in Human Serum

            Weiqiang Chen, Guoqiang Bao, Lin Zhao, and Haichao Wang

 

3. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus

            Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, and Cong-Qiu Chu

 

4. Application of Immunohistochemistry in Basic and Clinical Studies

            Aihua Li and Dong-Hua Yang

 

5. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma

            Takaaki Kamatani

 

6. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum

            Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, and Petr Vodicka

 

7. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence

            Herui Wang, Ryan L. Pangilinan, and Yan Zhu

 

Part II: Cytokine Bioassays

 

8. Analysis of IFNg-Induced Migration of Ovarian Cancer Cells

            Bijaya Gaire, Mohammad M. Uddin, Yue Zou, and Ivana Vancurova

 

9. Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells

            Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, and Ivana Vancurova

 

10. Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids

            Mohammad M. Uddin, Bijaya Gaire, and Ivana Vancurova

 

11. Detection of Ferroptosis by BODIPY™ 581/591 C11

            Alejandra M. Martinez, Ahryun Kim, and Wan Seok Yang

 

12. Methods for Studying TNFα-Induced Autophagy

            Sheyda Najafi, Ehab M. Abo-Ali, and Vikas V. Dukhande

 

13. Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting

            Andy Qingan Yuan

 

14. Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry

            Chong Xu and Yibo Yin

 

15. Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells

            Wenjun Wu, Zhihui Xiao, Deon Buritis, and Vladimir Poltoratsky

 

16. Single-Stranded DNA Aptamers against TNF and Their Potential Applications

            Shao Tao, Song Pingfang, Xiaowei Zhang, Lingshu Zhang, and Cong-Qiu Chu

 

17. Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells

            Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad Uddin, Yan Zhu, and Ales Vancura

 

Part III: Expression and Regulation of Immune Mediators in Cancer Cells

 

18. Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA

            Sveta Padmanabhan, Yue Zou, and Ivana Vancurova

 

19. Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells

            Sveta Padmanabhan, Yue Zou, and Ivana Vancurova

 

20. Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation

            Yue Zou, Sveta Padmanabhan, and Ivana Vancurova

 

21. Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer

            Ruizhi Wang, Md. Faruk Hossain, Jovan Mirkovic, Samuel Sabzanov, and Matteo Ruggiu

 

22. Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells

            Julian J. Freen-van Heeren, Benoit P. Nicolet, and Monika C. Wolkers

 

23. Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes

            Kiersten P. Carter, Jeffrey E. Segall, and Dianne Cox

 

24. Optogenetics: Rho GTPases Activated by Light in Living Macrophages

            Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, and Louis Hodgson

 

Part IV: Bioinformatics and Genome Editing Approaches

 

25. High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer

            Susumu Rokudai

 

26. Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis

            Vitalay Fomin and Carol Prives

 

27. Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees

            Juan C. Santos

 

28. Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing

            Olivia Hu, Alessandro Provvido, and Yan Zhu

 

29. Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9

            Zijun Huo, Jian Tu, Dung-Fang Lee, and Ruiying Zhao

Blurb

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.