Hypertension: Methods and Protocols

ISBN-13: 9781493982530 / Angielski / Miękka / 2018 / 437 str.

Rhian M. Touyz; Ernesto L. Schiffrin

Spis treści

Large Scale Transcriptome Analysis.- Methods to Assess Genetic Risk Prediction.- Microarray Analysis of Hypertension.- Tissue Proteomics in Vascular Disease.- Urine Metabolomics in Hypertension Research.- Systems Biology Approach in Hypertension Research.- Measurement of Angiotensin Peptides: HPLC, RIA.- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2).- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate.- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays.- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes.- Dopaminergic Immunofluorescence Studies in Kidney Tissue.- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps).- Intracellular Free Calcium Measurement Using Confocal Imaging.- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells.- In Vitro Analysis of Hypertensive Signal Transduction:  Kinase Activation, Kinase Manipulation, and Physiologic Outputs.- In vitro and in vivo Approaches to Assess Rho-kinase Activity.- NADPH Oxidases and Measurement of Reactive Oxygen Species.- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation.- Assessment of Caveolae/Lipid Rafts in Isolated Cells.- Isolation and Characterization of Circulating Microparticles by Flow Cytometry.- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR.- Isolation and Differentiation of Murine Macrophages.- Isolation and Differentiation of Human Macrophages.- Isolation of Immune Cells for Adoptive Transfer.- Isolation and Culture of Endothelial Cells from Large Vessels.- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels.- Evaluation of Endothelial Dysfunction in vivo.- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions.- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ.- Renal Delivery of Anti-microRNA Oligonucleotides in Rats.- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs. 

Blurb

This volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on genetics and mmics of hypertension; the Renin-Angiotensin-Aldosterone System; vasoactive agents and hypertension; signal transduction and reactive oxygen species; novel cell models and approaches to study molecular mechanisms of hypertension; vascular physiology; and new approaches to manipulate mouse models to study molecular mechanisms of hypertension. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Hypertension: Methods and Protocols aims to ensure successful results in the further study of this vital field.