Human Pluripotent Stem Cells: Methods and Protocols

ISBN-13: 9781617792007 / Angielski / Twarda / 2011 / 468 str.

Philip H. Schwartz; Robin Wesselschmidt

Opis

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

Recenzje

From the reviews:

"The volume is a compilation of 33 detailed protocols divided into six parts ... . All the protocols are superbly illustrated with many figures and charts presented in full colours. I think this book is a must for all those are working with human pluripotent stem cells, no matter if students entering the field or colleagues already involved, each of them will get the chance to sharpen their conceptual insights." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)

Spis treści

Section I. Laboratory Essentials

1. The Stem Cell Laboratory: Design, Equipment, and Oversight

                Robin L. Wesselschmidt and Philip H. Schwartz

 

2. Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research

                Lesley Young, Lyn Healy, and Glyn N. Stacey

 

Section II.  Derivation

 

3. Embryonic Stem Cell Derivation from Human Embryos

                Paul Lerou

 

4. Derivation of Human Parthenogenetic Stem Cell Lines

                Nikolay Turovets, Andrey Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa

Agapova, and Elena Revazova

 

5. Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer

                Justine D. Miller and Thorsten M. Schlaeger

 

6. Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction

                Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz

 

7. Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons

                Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, and Andras Nagy

 

 

Section III.  Growth, Maintenance and Expansion

 

8. Traditional Human Embryonic Stem Cell Culture

                Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, and Alexander E. Stover

 

9. Xeno-Free Culture of Human Pluripotent Stem Cells

                Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta

10. Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging

                Alexander E. Stover and Philip H. Schwartz

 

11. GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications

                Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture

 

12. Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging          

                Victoria Fox

 

 

Section IV. Characterization

 

13. Classical Cytogenetics: Karyotyping Techniques

                Steven E. Bates

 

14. FISH Analysis of Human Pluripotent Stem Cells

                Suzanne E. Peterson, Jerold Chun, and Jeanne Loring

 

15. Immunocytochemical Analysis of Human Pluripotent Stem Cells

                Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz

 

16. Flow Cytometric Analysis of Human Pluripotent Stem Cells

                Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett

 

17. The Teratoma Assay: An In vivo Assessment of Pluripotency

                Robin L. Wesselschmidt

 

18. Detection of Copy Number Variation using SNP Genotyping

                Gregory M. Cooper and Heather C. Mefford

 

19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq

                Michael J. Hitchler and Judd C. Rice

20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays

                Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring

 

21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells

                Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle

 

22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells  

                Javier Muñoz and Albert J. R. Heck

 

 

Section V. Genetic Manipulation (Gene Modulation)

 

23. Lentivirus-mediated Modification of Pluripotent Stem Cells

                Ruchi Bajpai

 

24. Nucleofection of Human Embryonic Stem Cells

                Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan

 

25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

                Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice

 

26. Gene Targeting in Human Pluripotent Stem Cells

                Ying Liu and Mahendra Rao

 

27. Episomal Transgene Expression in Pluripotent Stem Cells

                Michele M.P. Lufino, Anna R. Popplestone, Sally A. Cowley, Pauline A.H. Edser, William S. James, and Richard Wade-Martins

 

 

Section VI:  Differentiation

 

28. The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures

                Alexander E. Stover and Philip H. Schwartz

 

29. Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells

                Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead

 

30. Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells

                Lixiang Ma, Yan Liu, and Su-Chun Zhang

 

31. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells

                Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme

 

32. In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells

                Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman

 

33. Differentiation of Dendritic Cells from Human Embryonic Stem Cells

                Kathryn M. Silk, Su-Yi Tseng, Kevin P. Nishimoto, Jane S. Lebkowski, Anita Reddy, and Paul J. Fairchild

Blurb

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.  Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

            Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.